Genome location: Pf3D7_14_v3:1,548,072..1,557,053(+)
Genome classification: Core
Product Description: metacaspase-2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0008234 | cysteine-type peptidase activity |
| Function | GO:0008233 | peptidase activity |
| Process | GO:0006915 | apoptotic process |
| Process | GO:0006508 | proteolysis |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.23 | 0.89 |
| Trophozoite | expressed | 0.63 | 0.65 |
| Schizont | expressed | 1.64 | 0.33 |
| Gametocyte | expressed | 0.89 | 0.53 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1438400
Resistome Missense Mutations: N669H
Resistome Compounds with Missense Mutations: MMV676881
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.31 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1302300)
Modification: Disrupted | RMgm-5182
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL84
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 93 | 65 | 127 |
| disruptive | 100 | 317 | 161 | 412 |
| missense | 68 | 288 | 147 | 369 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 35 | 210 | 91 | 144 |
| disruptive | 186 | 677 | 298 | 677 |
| missense | 129 | 572 | 255 | 430 |
PlasmoDB Total SNPs: 425
Non-coding: 198 | Synonymous: 117 | Nonsynonymous: 110 | Stop Codon: 0
Protein Length: 2020 | Molecular Weight (kDa): 237.831
UniProt ID(s): Q8IL84
PDB ID(s): None
Isoelectric Point: 9.65
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17492602 | Features of apoptosis in Plasmodium falciparum erythrocytic stage through aputative role of PfMCA1 metacaspase-like protein. | Meslin B, Barnadas C, ..., Picot S | 10.1086/518253 |
| 29317266 | Biochemical characterization of unusual cysteine protease of P. falciparum,metacaspase-2 (MCA-2). | Vandana, Singh AP, ..., Pandey KC | 10.1016/j.molbiopara.2018.01.001 |
| 31896710 | A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malariaparasites. | Birnbaum J, Scharf S, ..., Spielmann T | 10.1126/science.aax4735 |
| 32202613 | A nonpeptidyl molecule modulates apoptosis-like cell death by inhibiting P.falciparum metacaspase-2. | Vandana, Shankar S, ..., Pandey KC | 10.1042/BCJ20200050 |
| 38039338 | The Kelch13 compartment contains highly divergent vesicle trafficking proteins inmalaria parasites | Schmidt S, Wichers-Misterek JS, ..., Spielmann T | 10.1371/journal.ppat.1011814 |