Genome location: Pf3D7_14_v3:1,575,439..1,577,046(+)
Genome classification: Core
Product Description: thioredoxin peroxidase 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.11.1.15 (Transferred entry: 1.11.1.24)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005730 | nucleolus |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0016209 | antioxidant activity |
| Function | GO:0003690 | double-stranded DNA binding |
| Function | GO:0003725 | double-stranded RNA binding |
| Function | GO:0106222 | long noncoding RNA binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0046983 | protein dimerization activity |
| Function | GO:0008379 | thioredoxin peroxidase activity |
| Process | GO:0042262 | DNA protection |
| Process | GO:0045454 | cell redox homeostasis |
| Process | GO:0006457 | protein folding |
| Process | GO:0009408 | response to heat |
| Process | GO:0006979 | response to oxidative stress |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.78 | 0.66 |
| Trophozoite | expressed | 1.23 | 0.41 |
| Schizont | expressed | 0.14 | 0.91 |
| Gametocyte | expressed | 0.97 | 0.52 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1438900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.848 | MFS: -2.296 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1430800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1302800)
Modification: Disrupted | RMgm-3411
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL80
"Best" AlphaFill ligand hit: DTU ((2r,3s)-1,4-dimercaptobutane-2,3-diol, Local RMSD=0.03) with 3SBC (Global RMSD=1.24)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.11.1.15 | peroxiredoxin | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q06830 | Peroxiredoxin-1 | OMA, OrthoDB | CHEMBL574646 |
Ortholog Group (OrthoMCL): OG6_100111
Most Similar Human Ortholog: Q06830
TM-align score: 0.97 | RMSD: 1.09
Seq Identity: 0.45 | Length: 194 / 195
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000117450 | peroxiredoxin 1 |
| ENSG00000117592 | peroxiredoxin 6 |
| ENSG00000123131 | peroxiredoxin 4 |
| ENSG00000165672 | peroxiredoxin 3 |
| ENSG00000167815 | peroxiredoxin 2 |
Protein Length: 195 | Molecular Weight (kDa): 21.807
PDB ID(s): None
Isoelectric Point: 7.1
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000866 | Alkyl hydroperoxide reductase subunit C/ Thiol specific antioxidant |
| InterPro | IPR019479 | Peroxiredoxin, C-terminal |
| InterPro | IPR024706 | Peroxiredoxin, AhpC-type |
| InterPro | IPR036249 | Thioredoxin-like superfamily |
| PFam | PF00578 | Alkyl hydroperoxide reductase subunit C/ Thiol specific antioxidant |
| PFam | PF10417 | Peroxiredoxin, C-terminal |
| Superfamily | SSF52833 | Thioredoxin-like superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 10 | 1 | 11 |
| disruptive | 0 | 2 | 6 | 11 |
| missense | 0 | 2 | 5 | 9 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 17 | 2 | 17 |
| disruptive | 1 | 9 | 14 | 37 |
| missense | 0 | 9 | 13 | 22 |
PlasmoDB Total SNPs: 81
Non-coding: 79 | Synonymous: 2 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11231293 | Thioredoxin peroxidases of the malarial parasite Plasmodium falciparum. | Rahlfs S, Becker K | 10.1046/j.1432-1327.2001.02005.x |
| 11295184 | Isolation and functional analysis of two thioredoxin peroxidases (peroxiredoxins)from Plasmodium falciparum. | Krnajski Z, Walter RD, Muller S | 10.1016/s0166-6851(01)00219-5 |
| 11463468 | Molecular characterization of a 2-Cys peroxiredoxin from the human malariaparasite Plasmodium falciparum. | Kawazu S, Komaki K, ..., Kano S | 10.1016/s0166-6851(01)00308-5 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 18692528 | 5' sequence- and chromatin modification-dependent gene expression in Plasmodiumfalciparum erythrocytic stage. | Komaki-Yasuda K, Okuwaki M, ..., Kawazu S | https://pubmed.ncbi.nlm.nih.gov/18692528/ |
| 21203490 | Compartmentation of redox metabolism in malaria parasites. | Kehr S, Sturm N, ..., Becker K | 10.1371/journal.ppat.1001242 |
| 23201565 | 2-Cys peroxiredoxin of Plasmodium falciparum is involved in resistance to heatstress of the parasite. | Kimura R, Komaki-Yasuda K, Kawazu S, Kano S | 10.1016/j.parint.2012.11.005 |
| 25092384 | Plasmodium knowlesi thioredoxin peroxidase 1 binds to nucleic acids and has RNAchaperone activity. | Hakimi H, Suganuma K, ..., Kawazu S | 10.1007/s00436-014-4060-0 |
| 28759593 | Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodiumvivax salivary gland sporozoites. | Swearingen KE, Lindner SE, ..., Mikolajczak SA | https://pubmed.ncbi.nlm.nih.gov/28759593/ |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31537845 | The interactome of 2-Cys peroxiredoxins in Plasmodium falciparum. | Brandstaedter C, Delahunty C, ..., Becker K | 10.1038/s41598-019-49841-3 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 35939693 | A nuclear redox sensor modulates gene activation and var switching in Plasmodiumfalciparum. | Heinberg A, Amit-Avraham I, ..., Dzikowski R | 10.1073/pnas.2201247119 |