PF3D7_1439100

Genome location: Pf3D7_14_v3:1,579,582..1,588,900(+)

Genome classification: Core

Function and Localization

Product Description: DEAD/DEAH box helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0003724	RNA helicase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.29	0.86
Trophozoite	expressed	0.67	0.62
Schizont	expressed	0.20	0.87
Gametocyte	possibly expressed	0.56	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1439100

Resistome Missense Mutations: N415Y, L1625M

Resistome Compounds with Missense Mutations: MMV1081413, MMV665852, OSM-S-106

Resistome # Samples with Disruptive Mutations: 8 (8 missense, 7 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.588 | #Insertions: 10

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1303000)

Relative Growth Rate: 0.89 ± 0.14
Confidence: 5.26

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1303000)

Modification: Disrupted | RMgm-3412

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL78

"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.02) with 6QV4 (Global RMSD=3.36)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O75643	U5 small nuclear ribonucleoprotein 200 kDa helicase		HOGENOM, OrthoDB	CHEMBL4060106CHEMBL4065018CHEMBL4070179CHEMBL4097764...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100305

Most Similar Human Ortholog: Q8N3C0

TM-align score: 0.72 | RMSD: 4.93

Seq Identity: 0.33 | Length: 1729 / 2472

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000112249	activating signal cointegrator 1 complex subunit 3
ENSG00000144028	small nuclear ribonucleoprotein U5 subunit 200

Protein Information

Protein Length: 2472 | Molecular Weight (kDa): 292.299

UniProt ID(s): Q8IL78

PDB ID(s): None

Isoelectric Point: 6.87

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR003593	AAA+ ATPase domain
InterPro	IPR004179	Sec63 domain
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR014756	Immunoglobulin E-set
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR036390	Winged helix DNA-binding domain superfamily
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
PFam	PF02889	Sec63 domain
Superfamily	SSF158702	Winged helix DNA-binding domain superfamily
Superfamily	SSF46785	P-loop containing nucleoside triphosphate hydrolase
Superfamily	SSF52540	Immunoglobulin E-set
Superfamily	SSF81296

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	16	111	73	165
disruptive	49	169	110	328
missense	35	152	106	304

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	29	247	103	156
disruptive	71	383	208	423
missense	50	333	195	367

PlasmoDB Total SNPs: 246

Non-coding: 105 | Synonymous: 86 | Nonsynonymous: 55 | Stop Codon: 0