PF3D7_1439700

Genome location: Pf3D7_14_v3:1,613,546..1,617,193(-)

Genome classification: Core

Function and Localization

Product Description: leucine carboxyl methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.233 ([Phosphatase 2A protein]-leucine-carboxy methyltransferase);2.3.1.231 (tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.05	0.97
Trophozoite	expressed	0.47	0.71
Schizont	expressed	0.12	0.91
Gametocyte	not expressed	0.34	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1439700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.182 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1303600)

Relative Growth Rate: 0.06 ± 0.13
Confidence: 5.43

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL72

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.77) with 3IEI (Global RMSD=2.14)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.-	Ex: homocysteine S-methyltransferase	EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate...
2.1.1.233	[phosphatase 2A protein]-leucine-carboxy methyltransferase	alpha-synucleinS-adenosyl-L-homocysteine
2.3.1.231	tRNAPhe {7-[3-amino-3-(methoxycarbonyl)propyl]wyosine37-N}-methoxycarbonyltransferase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100931

Most Similar Human Ortholog: O60294

TM-align score: 0.93 | RMSD: 1.71

Seq Identity: 0.32 | Length: 319 / 327

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000168806	leucine carboxyl methyltransferase 2
ENSG00000205629	leucine carboxyl methyltransferase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 122

Non-coding: 115 | Synonymous: 4 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 327 | Molecular Weight (kDa): 39.136

UniProt ID(s): Q8IL72

PDB ID(s): None

Isoelectric Point: 5.78

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007213	Methyltransferase Ppm1/Ppm2/Tcmp
InterPro	IPR016651	Leucine carboxyl methyltransferase 1
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF04072	Methyltransferase Ppm1/Ppm2/Tcmp
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase