Genome location: Pf3D7_14_v3:1,621,742..1,624,074(+)
Genome classification: Core
Product Description: triosephosphate isomerase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.3.1.1 (Triose-phosphate isomerase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0005829 | cytosol |
Function | GO:0004807 | triose-phosphate isomerase activity |
Process | GO:0006633 | fatty acid biosynthetic process |
Process | GO:0006094 | gluconeogenesis |
Process | GO:0046166 | glyceraldehyde-3-phosphate biosynthetic process |
Process | GO:0019563 | glycerol catabolic process |
Process | GO:0006096 | glycolytic process |
Process | GO:0006098 | pentose-phosphate shunt |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | expressed | N/A | N/A |
Ring | expressed | 0.29 | 0.86 |
Trophozoite | expressed | 0.85 | 0.55 |
Schizont | expressed | 0.10 | 0.93 |
Gametocyte | expressed | 0.35 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1439900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.063 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1303800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7KQM0
"Best" AlphaFill ligand hit: 2PG (2-phosphoglyceric acid, Local RMSD=0.04) with 3UWV (Global RMSD=1.86)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
5.3.1.1 | triose-phosphate isomerase | ATPNEMPCMBGSSGSO42-AsO2-AsO43-citratesuraminDiamidearsenateAtrazine... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100657
Most Similar Human Ortholog: P60174
TM-align score: 0.96 | RMSD: 1.33
Seq Identity: 0.42 | Length: 247 / 248
All Human Orthologs (OrthoMCL):
Gene ID | Description |
---|---|
ENSG00000111669 | triosephosphate isomerase 1 |
Protein Length: 248 | Molecular Weight (kDa): 27.935
UniProt ID(s): A0A144A4G4, Q07412, Q7KQM0
PDB ID(s): None
Isoelectric Point: 6.35
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR000652 | Triosephosphate isomerase |
InterPro | IPR020861 | Triosephosphate isomerase, active site |
InterPro | IPR022896 | Triosephosphate isomerase, bacterial/eukaryotic |
InterPro | IPR035990 | Triosephosphate isomerase superfamily |
PFam | PF00121 | Triosephosphate isomerase |
Superfamily | SSF51351 | Triosephosphate isomerase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 5 | 13 | 14 | 22 |
disruptive | 2 | 18 | 7 | 30 |
missense | 2 | 18 | 7 | 28 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 8 | 45 | 13 | 18 |
disruptive | 3 | 52 | 25 | 74 |
missense | 3 | 46 | 17 | 47 |
PlasmoDB Total SNPs: 74
Non-coding: 66 | Synonymous: 4 | Nonsynonymous: 4 | Stop Codon: 0
PMID | Title | Authors | DOI/Link |
---|---|---|---|
7903426 | Cloning of the triosephosphate isomerase gene of Plasmodium falciparum andexpression in Escherichia coli. | Ranie J, Kumar VP, Balaram H | 10.1016/0166-6851(93)90062-3 |
12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
22126412 | The structure of the thioredoxin-triosephosphate isomerase complex providesinsights into the reversible glutathione-mediated regulation of triosephosphateisomerase. | Shahul HM, Sarma SP | 10.1021/bi201224s |
26353595 | Interaction of Silver Nanoparticles with Triosephosphate Isomerase from Human andMalarial Parasite (Plasmodium falciparum): A Comparative Study. | De Moor W, van Marwijk J, Wilhelmi BS, Whiteley CG | 10.1166/jbn.2015.2003 |