About Gene List

PF3D7_1440200 (SPP)

Genome location: Pf3D7_14_v3:1,638,717..1,647,251(+)

Genome classification: Core

Function and Localization

Product Description: stromal-processing peptidase, putative

SignalP Peptide: MLKSDVVLLLYILIINLICC

# Transmembrane Domains: 0

EC Numbers: 3.4.24.55 (Pitrilysin);3.4.24.56 (Insulysin);4.2.1.24 (Porphobilinogen synthase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0005829 cytosol
Function GO:0004222 metalloendopeptidase activity
Function GO:0004655 porphobilinogen synthase activity
Function GO:0008270 zinc ion binding
Process GO:0006783 heme biosynthetic process
Process GO:0006508 proteolysis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.13 0.94
Trophozoite possibly expressed 0.81 0.57
Schizont not expressed 0.18 0.89
Gametocyte not expressed 0.38 0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1440200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.995 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1304200)

  • Relative Growth Rate: 0.10 ± 0.05
  • Confidence: 7.42

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL67

"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.23) with 6A2O (Global RMSD=23.75)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.4.24.55 pitrilysin DTTEDTAPCMBEGTAZincovcysteineantipainbacitracinchymostatinphosphoramidonN-ethylmaleimideChelating agents...
3.4.24.56 insulysin ATPNEMDTTEDTAPCMBEGTAPMSFInsL3amylinnestinUb1-72Ub1-74...
4.2.1.24 porphobilinogen synthase BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_110270

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 14 85 50 116
disruptive 35 192 104 235
missense 32 189 101 215

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 30 164 82 124
disruptive 66 354 179 375
missense 56 327 160 299

PlasmoDB Total SNPs: 224

Non-coding: 108 | Synonymous: 73 | Nonsynonymous: 43 | Stop Codon: 0

Protein Information

Protein Length: 1560 | Molecular Weight (kDa): 185.59

UniProt ID(s): Q8IL67, Q8MVZ1

PDB ID(s): None

Isoelectric Point: 6.95

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001731 Delta-aminolevulinic acid dehydratase
InterPro IPR007863 Peptidase M16, C-terminal
InterPro IPR011249 Metalloenzyme, LuxS/M16 peptidase-like
InterPro IPR011765 Peptidase M16, N-terminal
PFam PF00675 Peptidase M16, N-terminal
PFam PF05193 Peptidase M16, C-terminal
Superfamily SSF63411 Metalloenzyme, LuxS/M16 peptidase-like

Associated Publications

PMID Title Authors DOI/Link
38430291 Characterization and Biological Activities of the Ulvan Polysaccharide-RichFraction Obtained from Ulva rigida and Ulva pseudorotundata and Their Potentialfor Pharmaceutical Application Favero Massocato T, Robles-Carnero V, ..., Lopez Figueroa FD 10.1007/s10126-024-10299-8