Genome location: Pf3D7_14_v3:1,638,717..1,647,251(+)
Genome classification: Core
Product Description: stromal-processing peptidase, putative
SignalP Peptide: MLKSDVVLLLYILIINLICC
# Transmembrane Domains: 0
EC Numbers: 3.4.24.55 (Pitrilysin);3.4.24.56 (Insulysin);4.2.1.24 (Porphobilinogen synthase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0020011 | apicoplast |
Component | GO:0005829 | cytosol |
Function | GO:0004222 | metalloendopeptidase activity |
Function | GO:0004655 | porphobilinogen synthase activity |
Function | GO:0008270 | zinc ion binding |
Process | GO:0006783 | heme biosynthetic process |
Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | not expressed | 0.13 | 0.94 |
Trophozoite | possibly expressed | 0.81 | 0.57 |
Schizont | not expressed | 0.18 | 0.89 |
Gametocyte | not expressed | 0.38 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1440200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.995 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1304200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL67
"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.23) with 6A2O (Global RMSD=23.75)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 14 | 85 | 50 | 116 |
disruptive | 35 | 192 | 104 | 235 |
missense | 32 | 189 | 101 | 215 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 30 | 164 | 82 | 124 |
disruptive | 66 | 354 | 179 | 375 |
missense | 56 | 327 | 160 | 299 |
PlasmoDB Total SNPs: 224
Non-coding: 108 | Synonymous: 73 | Nonsynonymous: 43 | Stop Codon: 0
Protein Length: 1560 | Molecular Weight (kDa): 185.59
PDB ID(s): None
Isoelectric Point: 6.95
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR001731 | Delta-aminolevulinic acid dehydratase |
InterPro | IPR007863 | Peptidase M16, C-terminal |
InterPro | IPR011249 | Metalloenzyme, LuxS/M16 peptidase-like |
InterPro | IPR011765 | Peptidase M16, N-terminal |
PFam | PF00675 | Peptidase M16, N-terminal |
PFam | PF05193 | Peptidase M16, C-terminal |
Superfamily | SSF63411 | Metalloenzyme, LuxS/M16 peptidase-like |
PMID | Title | Authors | DOI/Link |
---|---|---|---|
38430291 | Characterization and Biological Activities of the Ulvan Polysaccharide-RichFraction Obtained from Ulva rigida and Ulva pseudorotundata and Their Potentialfor Pharmaceutical Application | Favero Massocato T, Robles-Carnero V, ..., Lopez Figueroa FD | 10.1007/s10126-024-10299-8 |