PF3D7_1440200 (SPP)

Genome location: Pf3D7_14_v3:1,638,717..1,647,251(+)

Genome classification: Core

Function and Localization

Product Description: stromal-processing peptidase, putative

SignalP Peptide: MLKSDVVLLLYILIINLICC

# Transmembrane Domains: 0

EC Numbers: 3.4.24.55 (Pitrilysin);3.4.24.56 (Insulysin);4.2.1.24 (Porphobilinogen synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005829	cytosol
Function	GO:0004222	metalloendopeptidase activity
Function	GO:0004655	porphobilinogen synthase activity
Function	GO:0008270	zinc ion binding
Process	GO:0006783	heme biosynthetic process
Process	GO:0006508	proteolysis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.13	0.94
Trophozoite	possibly expressed	0.81	0.57
Schizont	not expressed	0.18	0.89
Gametocyte	not expressed	0.38	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1440200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.995 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1304200)

Relative Growth Rate: 0.10 ± 0.05
Confidence: 7.42

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL67

"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.23) with 6A2O (Global RMSD=23.75)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.24.55	pitrilysin	DTTEDTAPCMBEGTAZincovcysteineantipainbacitracinchymostatinphosphoramidonN-ethylmaleimideChelating agents...
3.4.24.56	insulysin	ATPNEMDTTEDTAPCMBEGTAPMSFInsL3amylinnestinUb1-72Ub1-74...
4.2.1.24	porphobilinogen synthase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_110270

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	85	50	116
disruptive	35	192	104	235
missense	32	189	101	215

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	30	164	82	124
disruptive	66	354	179	375
missense	56	327	160	299

PlasmoDB Total SNPs: 224

Non-coding: 108 | Synonymous: 73 | Nonsynonymous: 43 | Stop Codon: 0

Protein Information

Protein Length: 1560 | Molecular Weight (kDa): 185.59

UniProt ID(s): Q8IL67, Q8MVZ1

PDB ID(s): None

Isoelectric Point: 6.95

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001731	Delta-aminolevulinic acid dehydratase
InterPro	IPR007863	Peptidase M16, C-terminal
InterPro	IPR011249	Metalloenzyme, LuxS/M16 peptidase-like
InterPro	IPR011765	Peptidase M16, N-terminal
PFam	PF00675	Peptidase M16, N-terminal
PFam	PF05193	Peptidase M16, C-terminal
Superfamily	SSF63411	Metalloenzyme, LuxS/M16 peptidase-like