Genome location: Pf3D7_14_v3:1,638,717..1,641,760(+)
Genome classification: Core
Product Description: porphobilinogen synthase
SignalP Peptide: MLKSDVVLLLYILIINLICC
# Transmembrane Domains: 0
EC Numbers: 4.2.1.24 (Porphobilinogen synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Function | GO:0004655 | porphobilinogen synthase activity |
| Function | GO:0008270 | zinc ion binding |
| Process | GO:0006783 | heme biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | expressed | 0.07 | 0.95 |
| Schizont | possibly expressed | 0.02 | 0.99 |
| Gametocyte | not expressed | 0.10 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1440300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.141 | MFS: -2.951 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1304100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL68
"Best" AlphaFill ligand hit: SHF (levulinic acid, Local RMSD=0.35) with 1B4K (Global RMSD=7.79)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.2.1.24 | porphobilinogen synthase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101254
Most Similar Human Ortholog: P13716
TM-align score: 0.89 | RMSD: 1.66
Seq Identity: 0.30 | Length: 306 / 451
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000148218 | aminolevulinate dehydratase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 24 | 15 | 29 |
| disruptive | 7 | 27 | 26 | 63 |
| missense | 7 | 27 | 25 | 61 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 50 | 20 | 30 |
| disruptive | 14 | 72 | 52 | 100 |
| missense | 12 | 68 | 47 | 80 |
PlasmoDB Total SNPs: 156
Non-coding: 141 | Synonymous: 7 | Nonsynonymous: 8 | Stop Codon: 0
Protein Length: 451 | Molecular Weight (kDa): 53.188
UniProt ID(s): Q8IL68, Q8MTV7, Q8MYK5, Q9U0R8
PDB ID(s): None
Isoelectric Point: 8.52
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001731 | Delta-aminolevulinic acid dehydratase |
| InterPro | IPR030656 | Delta-aminolevulinic acid dehydratase, active site |
| PFam | PF00490 | Delta-aminolevulinic acid dehydratase |
| Superfamily | SSF51569 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15144063 | Enzymes for heme biosynthesis are found in both the mitochondrion and plastid ofthe malaria parasite Plasmodium falciparum. | Sato S, Clough B, Coates L, Wilson RJ | 10.1078/1434461000169 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 37071336 | Biochemical and molecular characterization of a novel porphobilinogen synthasefrom Corynebacterium glutamicum | Zhu D, Wu C, ..., Li W | 10.1007/s11274-023-03615-2 |