Genome location: Pf3D7_14_v3:1,665,064..1,668,100(+)
Genome classification: Core
Product Description: major facilitator superfamily domain-containing protein, putative
SignalP Peptide: N/A
# Transmembrane Domains: 12
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.03 | 0.99 |
| Trophozoite | expressed | 0.13 | 0.91 |
| Schizont | expressed | 0.05 | 0.96 |
| Gametocyte | expressed | 0.10 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1440800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.964 | MFS: -1.891 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1304700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PbANKA_1304700)
Modification: Tagged | RMgm-4343
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL62
"Best" AlphaFill ligand hit: TAM (tris(hydroxyethyl)aminomethane, Local RMSD=0.16) with 4CZ8 (Global RMSD=16.04)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_104565
Most Similar Human Ortholog: Q14728
TM-align score: 0.76 | RMSD: 3.22
Seq Identity: 0.16 | Length: 383 / 582
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000109736 | major facilitator superfamily domain containing 10 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 18 | 18 | 36 |
| disruptive | 24 | 66 | 30 | 60 |
| missense | 17 | 58 | 28 | 49 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 49 | 21 | 36 |
| disruptive | 45 | 122 | 52 | 133 |
| missense | 33 | 102 | 42 | 94 |
PlasmoDB Total SNPs: 190
Non-coding: 150 | Synonymous: 23 | Nonsynonymous: 17 | Stop Codon: 0
Protein Length: 582 | Molecular Weight (kDa): 68.09
UniProt ID(s): Q8IL62
PDB ID(s): None
Isoelectric Point: 7.79
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011701 | Major facilitator superfamily |
| InterPro | IPR036259 | MFS transporter superfamily |
| PFam | PF07690 | Major facilitator superfamily |
| Superfamily | SSF103473 | MFS transporter superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 28902970 | A genetic screen in rodent malaria parasites identifies five new apicoplastputative membrane transporters, one of which is essential in human malariaparasites | Sayers CP, Mollard V, ..., Goodman CD | 10.1111/cmi.12789 |
| 35404116 | PMRT1, a Plasmodium-Specific Parasite Plasma Membrane Transporter, Is Essentialfor Asexual and Sexual Blood Stage Development. | Wichers JS, Mesen-Ramirez P, ..., Strauss J | 10.1128/mbio.00623-22 |