Genome location: Pf3D7_14_v3:1,669,946..1,673,071(-)
Genome classification: Core
Product Description: cytoplasmic tRNA 2-thiolation protein 2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Function | GO:0016783 | sulfurtransferase activity |
| Process | GO:0034227 | tRNA thio-modification |
| Process | GO:0002143 | tRNA wobble position uridine thiolation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.31 | 0.85 |
| Trophozoite | not expressed | 0.34 | 0.80 |
| Schizont | possibly expressed | 0.04 | 0.97 |
| Gametocyte | not expressed | 0.18 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1441000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.826 | MFS: -2.936 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1304900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL60
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 25 | 12 | 26 |
| disruptive | 8 | 71 | 33 | 99 |
| missense | 8 | 69 | 32 | 91 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 44 | 26 | 34 |
| disruptive | 16 | 154 | 61 | 131 |
| missense | 14 | 140 | 52 | 104 |
PlasmoDB Total SNPs: 107
Non-coding: 83 | Synonymous: 13 | Nonsynonymous: 11 | Stop Codon: 0
Protein Length: 556 | Molecular Weight (kDa): 65.861
UniProt ID(s): Q8IL60
PDB ID(s): None
Isoelectric Point: 9.21
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR019407 | Cytoplasmic tRNA 2-thiolation protein 2 |
| PFam | N/A | N/A |
| Superfamily | SSF52402 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 37462076 | Ncs2* mediates in vivo virulence of pathogenic yeast through sulphur modificationof cytoplasmic transfer RNA | Alings F, Scharmann K, ..., Leidel SA | 10.1093/nar/gkad564 |