PF3D7_1441300

Genome location: Pf3D7_14_v3:1,681,167..1,690,747(+)

Genome classification: Core

Function and Localization

Product Description: serine/threonine protein kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:1903561	extracellular vesicle
Function	GO:0004674	protein serine/threonine kinase activity
Process	GO:0006468	protein phosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.46	0.79
Trophozoite	not expressed	0.52	0.73
Schizont	expressed	1.61	0.35
Gametocyte	expressed	0.85	0.61

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1441300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.165 | #Insertions: 5

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1305200)

Relative Growth Rate: 0.98 ± 0.04
Confidence: 8.03

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1305200)

Modification: Disrupted | RMgm-3430

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL57

"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=0.13) with 2VZ6 (Global RMSD=8.00)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_151391

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	31	95	65	138
disruptive	82	364	199	501
missense	70	338	187	448

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	41	197	109	168
disruptive	160	765	318	635
missense	123	664	292	505

PlasmoDB Total SNPs: 404

Non-coding: 190 | Synonymous: 113 | Nonsynonymous: 99 | Stop Codon: 2

Protein Information

Protein Length: 2247 | Molecular Weight (kDa): 263.794

UniProt ID(s): Q8IL57

PDB ID(s): None

Isoelectric Point: 8.98

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR008271	Serine/threonine-protein kinase, active site
InterPro	IPR011009	Protein kinase-like domain superfamily
InterPro	IPR018247	EF-Hand 1, calcium-binding site
PFam	PF00069	Protein kinase domain
Superfamily	SSF56112	Protein kinase-like domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
33608523	20S proteasomes secreted by the malaria parasite promote its growth.	Dekel E, Yaffe D, ..., Regev-Rudzki N	10.1038/s41467-021-21344-8