Genome location: Pf3D7_14_v3:1,700,587..1,702,266(+)
Genome classification: Core
Product Description: mitochondrial inner membrane protease ATP23, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.24.- (Metalloendopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031314 | extrinsic component of mitochondrial inner membrane |
| Component | GO:0005758 | mitochondrial intermembrane space |
| Component | GO:0005739 | mitochondrion |
| Process | GO:0034982 | mitochondrial protein processing |
| Process | GO:0033615 | mitochondrial proton-transporting ATP synthase complex assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.03 | 0.98 |
| Trophozoite | possibly expressed | 0.25 | 0.84 |
| Schizont | possibly expressed | 0.04 | 0.97 |
| Gametocyte | expressed | 0.41 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1441700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.233 | MFS: -3.322 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1305600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1305600)
Modification: Disrupted | RMgm-3432
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL53
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.24.- | Ex: meprin A | GSHSDSATPADPNEMDTTPTXTNFEDTAEGTAPMSFPCMB... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102968
Most Similar Human Ortholog: Q9Y6H3
TM-align score: 0.68 | RMSD: 3.68
Seq Identity: 0.25 | Length: 201 / 458
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000166896 | ATP23 metallopeptidase and ATP synthase assembly factor homolog |
| ENSG00000262145 | ATP23 metallopeptidase and ATP synthase assembly factor homolog |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 17 | 15 | 25 |
| disruptive | 7 | 41 | 34 | 78 |
| missense | 7 | 35 | 31 | 74 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 40 | 16 | 30 |
| disruptive | 14 | 113 | 54 | 106 |
| missense | 10 | 92 | 45 | 81 |
PlasmoDB Total SNPs: 49
Non-coding: 19 | Synonymous: 23 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 458 | Molecular Weight (kDa): 54.967
UniProt ID(s): Q8IL53
PDB ID(s): None
Isoelectric Point: 9.31
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR019165 | Peptidase M76, ATP23 |
| PFam | PF09768 | Peptidase M76, ATP23 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 33956347 | Atp23p and Atp10p coordinate to regulate the assembly of yeast mitochondrial ATPsynthase | Yang G, Ding Y, ..., Zeng X | 10.1096/fj.202002475R |
| 20194738 | A single mutation in the first transmembrane domain of yeast COX2 enables itsallotopic expression | Supekova L, Supek F, Greer JE, Schultz PG | 10.1073/pnas.1000735107 |
| 35216443 | T1121G Point Mutation in the Mitochondrial Gene COX1 Suppresses a Null Mutationin ATP23 Required for the Assembly of Yeast Mitochondrial ATP Synthase | Yang G, Zhao T, ..., Zeng X | 10.3390/ijms23042327 |
| 35185991 | Protein Processing in Plant Mitochondria Compared to Yeast and Mammals | Heidorn-Czarna M, Maziak A, Janska H | 10.3389/fpls.2022.824080 |