About Gene List

PF3D7_1441900 (TFB5)

Genome location: Pf3D7_14_v3:1,704,178..1,705,128(+)

Genome classification: Core

Function and Localization

Product Description: RNA polymerase II transcription factor B subunit 5, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005669 transcription factor TFIID complex
Component GO:0000439 transcription factor TFIIH core complex
Component GO:0005675 transcription factor TFIIH holo complex
Process GO:0006289 nucleotide-excision repair
Process GO:0006294 nucleotide-excision repair, preincision complex assembly
Process GO:0070816 phosphorylation of RNA polymerase II C-terminal domain
Process GO:0006366 transcription by RNA polymerase II

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 0.02 0.99
Trophozoite possibly expressed 0.05 0.97
Schizont possibly expressed 0.02 0.99
Gametocyte possibly expressed 0.05 0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1441900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.122 | MFS: 0 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL51

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.7.6 DNA-directed RNA polymerase BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104325

Most Similar Human Ortholog: Q6ZYL4

TM-align score: 0.67 | RMSD: 2.32

Seq Identity: 0.33 | Length: 61 / 67

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000272047 general transcription factor IIH subunit 5

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 0 1 0 0
disruptive 0 0 0 0
missense 0 0 0 0

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 0 1 0 0
disruptive 0 0 0 0
missense 0 0 0 0

PlasmoDB Total SNPs: 46

Non-coding: 45 | Synonymous: 1 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 67 | Molecular Weight (kDa): 7.855

UniProt ID(s): M9VU17, Q8IL51

PDB ID(s): None

Isoelectric Point: 4.37

Protein Domain Annotations:

Source Family ID Description
InterPro IPR009400 TFIIH subunit TTDA/Tfb5
InterPro IPR035935 TFB5-like superfamily
PFam PF06331 TFIIH subunit TTDA/Tfb5
Superfamily SSF142897 TFB5-like superfamily

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
20606254 Structure determination of the minimal complex between Tfb5 and Tfb2, twosubunits of the yeast transcription/DNA-repair factor TFIIH: a retrospectivestudy Kainov DE, Cura V, ..., Poterszman A 10.1107/S0907444910009844
17409062 Suppression of the Schizosaccharomyces pombe cut12.1 cell-cycle defect bymutations in cdc25 and genes involved in transcriptional and translationalcontrol Tallada VA, Bridge AJ, Emery PA, Hagan IM 10.1534/genetics.107.072090
17215295 Tfb5 interacts with Tfb2 and facilitates nucleotide excision repair in yeast Zhou Y, Kou H, Wang Z 10.1093/nar/gkl1085
19897425 Interacting partners of the Tfb2 subunit from yeast TFIIH Kainov DE, Selth LA, ..., Poterzsman A 10.1016/j.dnarep.2009.10.002
17644494 Tfb5 is partially dispensable for Rad26 mediated transcription coupled nucleotideexcision repair in yeast Ding B, Ruggiero C, Chen X, Li S 10.1016/j.dnarep.2007.06.001