PF3D7_1442100 (RPA3)

Genome location: Pf3D7_14_v3:1,706,027..1,707,710(-)

Genome classification: Core

Function and Localization

Product Description: replication factor A protein 3, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005662	DNA replication factor A complex
Component	GO:0035861	site of double-strand break
Function	GO:0003684	damaged DNA binding
Function	GO:0003697	single-stranded DNA binding
Process	GO:0006260	DNA replication
Process	GO:0006284	base-excision repair
Process	GO:0000724	double-strand break repair via homologous recombination
Process	GO:0006298	mismatch repair
Process	GO:0006289	nucleotide-excision repair

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.05	0.97
Trophozoite	no data	0.46	0.72
Schizont	no data	0.10	0.93
Gametocyte	no data	0.25	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1442100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.566 | MFS: -3.267 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1306000)

Relative Growth Rate: 0.12 ± 0.17
Confidence: 4.97

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C6S3I6

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103793

Most Similar Human Ortholog: P35244

TM-align score: 0.85 | RMSD: 1.66

Seq Identity: 0.21 | Length: 105 / 109

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000106399	replication protein A3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	5	1	11
disruptive	0	4	5	12
missense	0	4	5	12

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	10	6	6
disruptive	1	13	5	25
missense	1	12	5	14

PlasmoDB Total SNPs: 87

Non-coding: 84 | Synonymous: 2 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 109 | Molecular Weight (kDa): 12.61

UniProt ID(s): C6S3I6

PDB ID(s): None

Isoelectric Point: 4.29

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR012340	Nucleic acid-binding, OB-fold
InterPro	IPR013970	Replication factor A protein 3
PFam	PF08661	Replication factor A protein 3
Superfamily	SSF50249	Nucleic acid-binding, OB-fold