PF3D7_1442300 (tRIP)

Genome location: Pf3D7_14_v3:1,712,163..1,714,843(-)

Genome classification: Core

Function and Localization

Product Description: tRNA import protein tRIP

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0009986	cell surface
Component	GO:0005634	nucleus
Component	GO:0005886	plasma membrane
Function	GO:0003723	RNA binding
Function	GO:0004812	aminoacyl-tRNA ligase activity
Process	GO:0006418	tRNA aminoacylation for protein translation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.82	0.62
Trophozoite	expressed	1.09	0.45
Schizont	expressed	0.21	0.86
Gametocyte	expressed	0.49	0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1442300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.417 | #Insertions: 2

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1306200)

Relative Growth Rate: 0.52 ± 0.14
Confidence: 5.34

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1306200)

Modification: Disrupted | RMgm-3435

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL48

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=2.00) with 4UP9 (Global RMSD=15.55)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P56192	Methionine--tRNA ligase, cytoplasmic	Homo sapiens	OrthoDB	CHEMBL308349CHEMBL308349CHEMBL72005CHEMBL72005...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100893

Most Similar Human Ortholog: Q12904

TM-align score: 0.61 | RMSD: 3.42

Seq Identity: 0.30 | Length: 219 / 402

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000164022	aminoacyl tRNA synthetase complex interacting multifunctional protein 1

Protein Information

Protein Length: 402 | Molecular Weight (kDa): 46.441

UniProt ID(s): Q8IL48

PDB ID(s): None

Isoelectric Point: 7.23

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002547	tRNA-binding domain
InterPro	IPR012340	Nucleic acid-binding, OB-fold
InterPro	IPR036282	Glutathione S-transferase, C-terminal domain superfamily
PFam	PF01588	tRNA-binding domain
Superfamily	SSF47616	Glutathione S-transferase, C-terminal domain superfamily
Superfamily	SSF50249	Nucleic acid-binding, OB-fold

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	21	14	32
disruptive	8	33	23	61
missense	8	32	23	59

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	42	24	45
disruptive	13	82	39	106
missense	13	76	38	81

PlasmoDB Total SNPs: 122

Non-coding: 101 | Synonymous: 12 | Nonsynonymous: 9 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
27071116	Apicomplexa-specific tRip facilitates import of exogenous tRNAs into malariaparasites.	Bour T, Mahmoudi N, ..., Frugier M	10.1073/pnas.1600476113
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596
33906926	Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH	Sinha A, Baumgarten S, ..., Scherf A	10.1128/mBio.00661-21
34530443	Identification of host tRNAs preferentially recognized by the Plasmodium surfaceprotein tRip.	Cela M, Theobald-Dietrich A, ..., Frugier M	10.1093/nar/gkab769