PF3D7_1442500

Genome location: Pf3D7_14_v3:1,724,029..1,727,809(-)

Genome classification: Core

Function and Localization

Product Description: geranylgeranyl transferase type-2 subunit alpha, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.5.1.60 (Protein geranylgeranyltransferase type II)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005968	Rab-protein geranylgeranyltransferase complex
Component	GO:0005737	cytoplasm
Function	GO:0004663	Rab geranylgeranyltransferase activity
Function	GO:0004661	protein geranylgeranyltransferase activity
Process	GO:0018344	protein geranylgeranylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	not expressed	0.34	0.79
Schizont	not expressed	0.03	0.98
Gametocyte	not expressed	0.12	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1442500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.967 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL46

"Best" AlphaFill ligand hit: FPP (farnesyl diphosphate, Local RMSD=0.04) with 3DSU (Global RMSD=2.31)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.5.1.60	protein geranylgeranyltransferase type II	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102100

Most Similar Human Ortholog: Q92696

TM-align score: 0.78 | RMSD: 4.27

Seq Identity: 0.22 | Length: 537 / 724

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000100949	Rab geranylgeranyltransferase subunit alpha
ENSG00000285193	Rab geranylgeranyltransferase subunit alpha

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	45	24	56
disruptive	32	108	70	160
missense	21	103	67	138

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	98	37	51
disruptive	51	248	99	248
missense	37	221	90	163

PlasmoDB Total SNPs: 170

Non-coding: 109 | Synonymous: 32 | Nonsynonymous: 29 | Stop Codon: 0

Protein Information

Protein Length: 724 | Molecular Weight (kDa): 87.483

UniProt ID(s): Q8IL46

PDB ID(s): None

Isoelectric Point: 7.79

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002088	Protein prenyltransferase, alpha subunit
InterPro	IPR032955	Geranylgeranyl transferase type-2 subunit alpha
PFam	PF01239	Protein prenyltransferase, alpha subunit
Superfamily	SSF48439	N/A
Superfamily	SSF52075