About Gene List

PF3D7_1442900 (ARFGEF)

Genome location: Pf3D7_14_v3:1,752,543..1,764,248(+)

Genome classification: Core

Function and Localization

Product Description: Sec7 domain-containing protein ARFGEF, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Process GO:0031001 response to brefeldin A

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 2.26 0.23
Trophozoite expressed 1.75 0.27
Schizont expressed 1.70 0.31
Gametocyte possibly expressed 1.34 0.38

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1442900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.05 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_129597

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 40 177 108 244
disruptive 169 520 257 571
missense 102 421 236 496

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 73 340 152 258
disruptive 285 913 411 728
missense 174 749 358 544

PlasmoDB Total SNPs: 472

Non-coding: 73 | Synonymous: 240 | Nonsynonymous: 158 | Stop Codon: 1

Protein Information

Protein Length: 3384 | Molecular Weight (kDa): 404.583

UniProt ID(s): Q8IL42

PDB ID(s): None

Isoelectric Point: 6.49

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000904 Sec7 domain
InterPro IPR016024 Armadillo-type fold
InterPro IPR035999 Sec7 domain superfamily
PFam PF01369 Sec7 domain
Superfamily SSF48371 Armadillo-type fold
Superfamily SSF48425 Sec7 domain superfamily

Associated Publications

PMID Title Authors DOI/Link
11555294 A point mutation in an unusual Sec7 domain is linked to brefeldin A resistance ina Plasmodium falciparum line generated by drug selection. Baumgartner F, Wiek S, ..., Lingelbach K 10.1046/j.1365-2958.2001.02572.x
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
15500915 Double cross-over gene replacement within the sec 7 domain of a GDP-GTP exchangefactor from Plasmodium falciparum allows the generation of a transgenic brefeldinA-resistant parasite line. Wiek S, Cowman AF, Lingelbach K 10.1016/j.molbiopara.2004.06.015
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
35474054 Involvement of Sec71 and Ubp2 in tunicamycin-induced ER stress response in thefission yeast Yemenici M, Kartal Sural B, Karaer Uzuner S, Palabiyik B 10.1007/s11033-022-07321-4