Genome location: Pf3D7_14_v3:1,763,820..1,768,781(-)
Genome classification: Core
Product Description: serine/threonine protein kinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004672 | protein kinase activity |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Function | GO:0004713 | protein tyrosine kinase activity |
| Process | GO:0018105 | peptidyl-serine phosphorylation |
| Process | GO:0018107 | peptidyl-threonine phosphorylation |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.98 |
| Trophozoite | expressed | 0.28 | 0.83 |
| Schizont | expressed | 0.65 | 0.62 |
| Gametocyte | expressed | 0.52 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1443000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.485 | #Insertions: 3
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1306900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1306900)
Modification: Disrupted | RMgm-3438
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL41
"Best" AlphaFill ligand hit: 3NG (5-[(3-chlorophenyl)amino]benzo[c][2,6]naphthyridine-8-carboxylic acid, Local RMSD=0.06) with 6FYP (Global RMSD=7.37)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q61214 | Dual specificity tyrosine-phosphorylation-regulated kinase 1A | Mus musculus | OrthoDB | CHEMBL37139CHEMBL81714CHEMBL377430GNF-PF-2722... |
| Q92630 | Dual specificity tyrosine-phosphorylation-regulated kinase 2 | Homo sapiens | OrthoDB | HS38US20230303553, Example 1US20230303553, Example 29US20230303553, Example 43... |
| Q9Y463 | Dual specificity tyrosine-phosphorylation-regulated kinase 1B | OrthoDB | US9446044, 81US9446044, 44US9446044, 45US9446044, 56... | |
| O43781 | Dual specificity tyrosine-phosphorylation-regulated kinase 3 | Homo sapiens | OrthoDB | CHEMBL3297832CHEMBL3589664CHEMBL3589666CHEMBL3589674... |
| Q13627 | Dual specificity tyrosine-phosphorylation-regulated kinase 1A | Homo sapiens | OrthoDB | NR93A55E45F4... |
| Q9NR20 | Dual specificity tyrosine-phosphorylation-regulated kinase 4 | Homo sapiens | OrthoDB | CHEMBL3103192CHEMBL1802358Harmine hydrochlorideG6416 (GW5074)... |
| Q63470 | Dual specificity tyrosine-phosphorylation-regulated kinase 1A | Rattus | OrthoDB | Epigallocatechin 3-gallatePurvalanol ARoscovitineCHEMBL373834... |
Protein Length: 1268 | Molecular Weight (kDa): 150.096
UniProt ID(s): Q8IL41
PDB ID(s): None
Isoelectric Point: 7.71
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 52 | 37 | 97 |
| disruptive | 38 | 159 | 115 | 248 |
| missense | 35 | 152 | 107 | 223 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 116 | 55 | 86 |
| disruptive | 76 | 355 | 174 | 340 |
| missense | 61 | 318 | 156 | 264 |
PlasmoDB Total SNPs: 175
Non-coding: 50 | Synonymous: 69 | Nonsynonymous: 56 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21312235 | Two nucleus-localized CDK-like kinases with crucial roles for malaria parasiteerythrocytic replication are involved in phosphorylation of splicing factor. | Agarwal S, Kern S, ..., Pradel G | 10.1002/jcb.23034 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |