Genome location: Pf3D7_14_v3:1,780,604..1,784,857(-)
Genome classification: Core
Product Description: poly(A)-specific ribonuclease PARN, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.13.4 (Poly(A)-specific ribonuclease)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0000175 | 3'-5'-exoribonuclease activity |
| Function | GO:0003723 | RNA binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.45 | 0.75 |
| Schizont | expressed | 0.06 | 0.97 |
| Gametocyte | expressed | 0.44 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1443500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.034 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1307400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL36
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.13.4 | poly(A)-specific ribonuclease | ATPADPAMPGTPGDPCMPGMPEDTAK3PO4eIF4Epoly(C)Poly(A)... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102215
Most Similar Human Ortholog: A0A494C156
TM-align score: 0.81 | RMSD: 3.31
Seq Identity: 0.21 | Length: 412 / 862
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000140694 | poly(A)-specific ribonuclease |
| ENSG00000274829 | poly(A)-specific ribonuclease |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 39 | 17 | 54 |
| disruptive | 14 | 73 | 47 | 127 |
| missense | 12 | 71 | 45 | 120 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 75 | 35 | 64 |
| disruptive | 31 | 184 | 95 | 179 |
| missense | 20 | 161 | 82 | 162 |
PlasmoDB Total SNPs: 149
Non-coding: 110 | Synonymous: 22 | Nonsynonymous: 17 | Stop Codon: 0
Protein Length: 862 | Molecular Weight (kDa): 102.257
UniProt ID(s): Q8IL36
PDB ID(s): None
Isoelectric Point: 7.72
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006941 | Ribonuclease CAF1 |
| InterPro | IPR012337 | Ribonuclease H-like superfamily |
| PFam | PF04857 | Ribonuclease CAF1 |
| Superfamily | SSF53098 | Ribonuclease H-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |