Genome location: Pf3D7_14_v3:1,791,629..1,793,782(-)
Genome classification: Core
Product Description: dephospho-CoA kinase
SignalP Peptide: N/A
# Transmembrane Domains: 3
EC Numbers: 2.7.1.24 (Dephospho-CoA kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0004140 | dephospho-CoA kinase activity |
| Process | GO:0015937 | coenzyme A biosynthetic process |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | expressed | 0.18 | 0.88 |
| Schizont | expressed | 0.14 | 0.89 |
| Gametocyte | not expressed | 0.05 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1443700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.234 | MFS: -3.436 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1307600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL34
"Best" AlphaFill ligand hit: BU2 (1,3-butanediol, Local RMSD=0.38) with 4TTR (Global RMSD=4.00)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.24 | dephospho-CoA kinase | CoACTPUTPEDC4acetyl-CoAcoenzyme Amalonyl-CoAdeoxycholate |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101308
Most Similar Human Ortholog: K7ESP4
TM-align score: 0.88 | RMSD: 1.79
Seq Identity: 0.26 | Length: 201 / 274
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000172992 | dephospho-CoA kinase domain containing |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 16 | 8 | 13 |
| disruptive | 1 | 3 | 2 | 9 |
| missense | 1 | 3 | 1 | 6 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 27 | 8 | 20 |
| disruptive | 8 | 12 | 18 | 41 |
| missense | 3 | 5 | 16 | 29 |
PlasmoDB Total SNPs: 119
Non-coding: 112 | Synonymous: 7 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 274 | Molecular Weight (kDa): 31.943
UniProt ID(s): Q8IL34
PDB ID(s): None
Isoelectric Point: 9.92
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001977 | Dephospho-CoA kinase |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF01121 | Dephospho-CoA kinase |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34031901 | Dephospho-CoA kinase, a nuclear-encoded apicoplast protein, remains active andessential after Plasmodium falciparum apicoplast disruption. | Swift RP, Rajaram K, Liu HB, Prigge ST | 10.15252/embj.2020107247 |
| 27855724 | Biological characterization of chemically diverse compounds targeting thePlasmodium falciparum coenzyme A synthesis pathway | Fletcher S, Lucantoni L, ..., Avery VM | 10.1186/s13071-016-1860-3 |
| 28676844 | Genetic Characterization of Coenzyme A Biosynthesis Reveals Essential DistinctiveFunctions during Malaria Parasite Development in Blood and Mosquito | Hart RJ, Abraham A, Aly ASI | 10.3389/fcimb.2017.00260 |
| 36314787 | Dephospho-Coenzyme A Kinase Is an Exploitable Drug Target against Plasmodiumfalciparum: Identification of Selective Inhibitors by High-Throughput Screeningof a Large Chemical Compound Library | Nurkanto A, Imamura R, ..., Nozaki T | 10.1128/aac.00420-22 |