Genome location: Pf3D7_14_v3:1,828,404..1,830,177(+)
Genome classification: Core
Product Description: 1-acyl-sn-glycerol-3-phosphate acyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 2.3.1.51 (1-acylglycerol-3-phosphate O-acyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0003841 | 1-acylglycerol-3-phosphate O-acyltransferase activity |
| Process | GO:0006654 | phosphatidic acid biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.17 | 0.91 |
| Trophozoite | expressed | 0.51 | 0.69 |
| Schizont | expressed | 0.48 | 0.69 |
| Gametocyte | expressed | 0.33 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1444300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.22 | MFS: -2.951 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_1312000)
Modification: Tagged | RMgm-980
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL28
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.51 | 1-acylglycerol-3-phosphate O-acyltransferase | EDTAMgCl2CaCl2CHAPSCI-976Triton X-100stearoyl-CoAPhospholipidsN-ethylmaleimide2-mercaptoethanolsodium deoxycholatelysophosphatidylcholine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100535
Most Similar Human Ortholog: O15120
TM-align score: 0.74 | RMSD: 2.94
Seq Identity: 0.17 | Length: 241 / 292
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000169692 | 1-acylglycerol-3-phosphate O-acyltransferase 2 |
| ENSG00000204310 | 1-acylglycerol-3-phosphate O-acyltransferase 1 |
| ENSG00000206324 | 1-acylglycerol-3-phosphate O-acyltransferase 1 |
| ENSG00000226467 | 1-acylglycerol-3-phosphate O-acyltransferase 1 |
| ENSG00000227642 | 1-acylglycerol-3-phosphate O-acyltransferase 1 |
| ENSG00000228892 | 1-acylglycerol-3-phosphate O-acyltransferase 1 |
| ENSG00000235758 | 1-acylglycerol-3-phosphate O-acyltransferase 1 |
| ENSG00000236873 | 1-acylglycerol-3-phosphate O-acyltransferase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 14 | 15 | 25 |
| disruptive | 3 | 11 | 8 | 24 |
| missense | 3 | 11 | 8 | 21 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 34 | 10 | 34 |
| disruptive | 6 | 36 | 24 | 76 |
| missense | 6 | 30 | 20 | 43 |
PlasmoDB Total SNPs: 84
Non-coding: 78 | Synonymous: 3 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 292 | Molecular Weight (kDa): 33.747
UniProt ID(s): Q8IL28
PDB ID(s): None
Isoelectric Point: 9.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002123 | Phospholipid/glycerol acyltransferase |
| PFam | PF01553 | Phospholipid/glycerol acyltransferase |
| Superfamily | SSF69593 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 24330260 | Enzymes involved in plastid-targeted phosphatidic acid synthesis are essentialfor Plasmodium yoelii liver-stage development. | Lindner SE, Sartain MJ, ..., Vaughan AM | 10.1111/mmi.12485 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 34982825 | Lysophosphatidic acid acyltransferases: a link with intracellular proteintrafficking in Arabidopsis root cells | Wattelet-Boyer V, Le Guedard M, ..., Moreau P | 10.1093/jxb/erab504 |