Genome location: Pf3D7_14_v3:1,843,368..1,846,989(-)
Genome classification: Core
Product Description: fructose-bisphosphate aldolase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.1.2.13 (Fructose-bisphosphate aldolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003779 | actin binding |
| Function | GO:0004332 | fructose-bisphosphate aldolase activity |
| Process | GO:0008154 | actin polymerization or depolymerization |
| Process | GO:0030388 | fructose 1,6-bisphosphate metabolic process |
| Process | GO:0006096 | glycolytic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 2.10 | 0.31 |
| Trophozoite | expressed | 2.55 | 0.09 |
| Schizont | expressed | 1.18 | 0.44 |
| Gametocyte | expressed | 1.04 | 0.51 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1444800
Resistome Missense Mutations: G177V
Resistome Compounds with Missense Mutations: Cipargamin/KAE609/NITD609
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.268 | MFS: -2.718 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1308600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7KQL9
"Best" AlphaFill ligand hit: G3H (glyceraldehyde-3-phosphate, Local RMSD=0.14) with 5TK3 (Global RMSD=3.22)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.1.2.13 | fructose-bisphosphate aldolase | ATPADPAMPKClIMPCysEDTAHgCl2MgCl2CaCl2CuSO4MnCl2... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P00883 | Fructose-bisphosphate aldolase A | Oryctolagus cuniculus | OMA, OrthoMCL BLAST | Phosphoglycolohydroxamic AcidCHEMBL258208CHEMBL2017785 |
Ortholog Group (OrthoMCL): OG6_100527
Most Similar Human Ortholog: A0A3B3IS80
TM-align score: 0.97 | RMSD: 1.10
Seq Identity: 0.51 | Length: 340 / 369
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000109107 | aldolase, fructose-bisphosphate C |
| ENSG00000136872 | aldolase, fructose-bisphosphate B |
| ENSG00000149925 | aldolase, fructose-bisphosphate A |
Protein Length: 369 | Molecular Weight (kDa): 40.105
UniProt ID(s): A0A144A3T1, A0A4Y5WWU3, A0A4Y5WWU6, A1BY85, B7SSG8, B7SSG9, B7SSH0, B7SSH1, P14223, Q27744, Q7KQL9, Q9U6R2
PDB ID(s): 4TR9
Isoelectric Point: 8.14
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000741 | Fructose-bisphosphate aldolase, class-I |
| InterPro | IPR029768 | Fructose-bisphosphate aldolase class-I active site |
| PFam | PF00274 | Fructose-bisphosphate aldolase, class-I |
| Superfamily | SSF51569 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 1 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 1 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 171
Non-coding: 164 | Synonymous: 4 | Nonsynonymous: 3 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 2190085 | Plasmodium falciparum aldolase: gene structure and localization. | Knapp B, Hundt E, Kupper HA | 10.1016/0166-6851(90)90074-v |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17426153 | Aldolase provides an unusual binding site for thrombospondin-related anonymousprotein in the invasion machinery of the malaria parasite. | Bosch J, Buscaglia CA, ..., Hol WG | 10.1073/pnas.0605301104 |
| 25261592 | Plasmodium falciparum aldolase and the C-terminal cytoplasmic domain of certainapical organellar proteins promote actin polymerization. | Diaz SA, Martin SR, ..., Holder AA | 10.1016/j.molbiopara.2014.09.006 |
| 26289816 | Inhibition by stabilization: targeting the Plasmodium falciparum aldolase-TRAPcomplex. | Nemetski SM, Cardozo TJ, ..., Bosch J | 10.1186/s12936-015-0834-9 |
| 27714937 | PlasmoSEP: Predicting surface-exposed proteins on the malaria parasite usingsemisupervised self-training and expert-annotated data. | El-Manzalawy Y, Munoz EE, Lindner SE, Honavar V | 10.1002/pmic.201600249 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |