PF3D7_1445100

Genome location: Pf3D7_14_v3:1,851,252..1,855,162(-)

Genome classification: Core

Function and Localization

Product Description: histidine--tRNA ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.1.1.21 (Histidine--tRNA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005829	cytosol
Component	GO:0005739	mitochondrion
Function	GO:0004812	aminoacyl-tRNA ligase activity
Function	GO:0004821	histidine-tRNA ligase activity
Process	GO:0006427	histidyl-tRNA aminoacylation
Process	GO:0032543	mitochondrial translation
Process	GO:0006418	tRNA aminoacylation for protein translation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.88	0.61
Trophozoite	expressed	0.73	0.60
Schizont	possibly expressed	0.20	0.87
Gametocyte	not expressed	0.33	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1445100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.131 | MFS: -3.15 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1308900)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IL22

"Best" AlphaFill ligand hit: HAM (histidyl-adenosine monophosphate, Local RMSD=1.12) with 3HRK (Global RMSD=4.96)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.1.1.21	histidine-tRNA ligase	ADPAMPKClNEMRNAiornithinediphosphateD-histidineL-histidinolDL-homocysteinequinolin-3-amineN-Acetylhistidine...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P12081	Histidine--tRNA ligase, cytoplasmic	Homo sapiens	OrthoDB	HisSA

Orthology Information

Ortholog Group (OrthoMCL): OG6_100351

Most Similar Human Ortholog: D6RJE6

TM-align score: 0.93 | RMSD: 1.56

Seq Identity: 0.48 | Length: 285 / 1132

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000112855	histidyl-tRNA synthetase 2, mitochondrial
ENSG00000170445	histidyl-tRNA synthetase 1

Protein Information

Protein Length: 1132 | Molecular Weight (kDa): 133.668

UniProt ID(s): Q8IL22

PDB ID(s): None

Isoelectric Point: 7.77

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004154	Anticodon-binding
InterPro	IPR041715	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain
PFam	PF03129	Anticodon-binding
PFam	PF13393	Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain
Superfamily	SSF52954	N/A
Superfamily	SSF55681

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	66	39	74
disruptive	23	147	73	175
missense	23	141	70	158

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	29	122	48	83
disruptive	41	293	116	241
missense	37	264	104	197

PlasmoDB Total SNPs: 111

Non-coding: 46 | Synonymous: 34 | Nonsynonymous: 31 | Stop Codon: 0