Genome location: Pf3D7_14_v3:1,855,451..1,859,752(+)
Genome classification: Core
Product Description: ATP-dependent RNA helicase MAK5, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005730 | nucleolus |
| Component | GO:0005634 | nucleus |
| Function | GO:0004386 | helicase activity |
| Process | GO:0042273 | ribosomal large subunit biogenesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.41 | 0.42 |
| Trophozoite | expressed | 1.05 | 0.47 |
| Schizont | possibly expressed | 0.24 | 0.86 |
| Gametocyte | expressed | 0.79 | 0.58 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1445200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.126 | MFS: -2.897 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1309000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL21
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.02) with 6L5M (Global RMSD=2.64)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102605
Most Similar Human Ortholog: A0A087WXU8
TM-align score: 0.73 | RMSD: 3.27
Seq Identity: 0.25 | Length: 397 / 796
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000089737 | DEAD-box helicase 24 |
| ENSG00000273761 | DEAD-box helicase 24 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 48 | 21 | 42 |
| disruptive | 30 | 124 | 84 | 157 |
| missense | 21 | 115 | 80 | 144 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 19 | 83 | 27 | 58 |
| disruptive | 54 | 260 | 132 | 208 |
| missense | 41 | 227 | 116 | 172 |
PlasmoDB Total SNPs: 201
Non-coding: 127 | Synonymous: 38 | Nonsynonymous: 34 | Stop Codon: 2
Protein Length: 796 | Molecular Weight (kDa): 94.124
UniProt ID(s): Q8IL21
PDB ID(s): None
Isoelectric Point: 9.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24312670 | Mak5 and Ebp2 act together on early pre-60S particles and their reduced functionality bypasses the requirement for the essential pre-60S factor Nsa1 | Pratte D, Singh U, Murat G, Kressler D | 10.1371/journal.pone.0082741 |
| 13680366 | Mak5p, which is required for the maintenance of the M1 dsRNA virus, is encoded bythe yeast ORF YBR142w and is involved in the biogenesis of the 60S subunit of theribosome | Zagulski M, Kressler D, ..., Herbert CJ | 10.1007/s00438-003-0913-4 |
| 35385737 | Emergence of the primordial pre-60S from the 90S pre-ribosome | Ismail S, Flemming D, ..., Hurt E | 10.1016/j.celrep.2022.110640 |
| 17248773 | Deletion of mitochondrial DNA bypassing a chromosomal gene needed for maintenanceof the killer plasmid of yeast | Wickner RB | 10.1093/genetics/87.3.441 |
| 30568249 | RNA helicases mediate structural transitions and compositional changes inpre-ribosomal complexes | Bruning L, Hackert P, ..., Bohnsack MT | 10.1038/s41467-018-07783-w |
| 16449635 | Comprehensive mutational analysis of yeast DEXD/H box RNA helicases involved inlarge ribosomal subunit biogenesis | Bernstein KA, Granneman S, ..., Baserga SJ | 10.1128/MCB.26.4.1195-1208.2006 |