Genome location: Pf3D7_14_v3:1,884,566..1,889,117(+)
Genome classification: Core
Product Description: ATP-dependent RNA helicase DDX17
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0033644 | host cell membrane |
| Component | GO:0005634 | nucleus |
| Component | GO:1990904 | ribonucleoprotein complex |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
| Function | GO:0004386 | helicase activity |
| Function | GO:0003676 | nucleic acid binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.67 | 0.67 |
| Trophozoite | expressed | 0.60 | 0.66 |
| Schizont | expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.60 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1445900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.276 | MFS: -3.019 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1441900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1309700)
Modification: Disrupted | RMgm-3450
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL13
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.05) with 5JCH (Global RMSD=9.76)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9HAZ1 | Dual specificity protein kinase CLK4 | Homo sapiens | OrthoDB | CHEMBL3297832CHEMBL3775393CHEMBL3774448CHEMBL3815054... |
| P49759 | Dual specificity protein kinase CLK1 | Homo sapiens | OrthoDB | US8546404, 6NR93A55E4... |
| P49760 | Dual specificity protein kinase CLK2 | Homo sapiens | OrthoDB | US9040694, 2US9040694, 3pyrazolo[1,5-a]pyrimidine 4bDorsomorphin... |
| O35492 | Dual specificity protein kinase CLK3 | Mus musculus | OrthoDB | CHEMBL1803078CHEMBL1803082CHEMBL1803083CHEMBL1803085... |
| O35491 | Dual specificity protein kinase CLK2 | Mus musculus | OrthoDB | CHEMBL3589660CHEMBL3589661CHEMBL3589662CHEMBL3589664... |
| O35493 | Dual specificity protein kinase CLK4 | Mus musculus | OrthoDB | CHEMBL3589660CHEMBL3589661CHEMBL3589662CHEMBL3589664... |
| P22518 | Dual specificity protein kinase CLK1 | Mus musculus | OrthoDB | MLS000518915CHEMBL2029615CHEMBL2029618CHEMBL4784564... |
| P49761 | Dual specificity protein kinase CLK3 | OrthoDB | 3A5CHEMBL3297832CHEMBL3774448CHEMBL3885335... | |
| Q5E175 | ATP-dependent RNA helicase RhlE | Plasmodium falciparum | OrthoMCL BLAST | Chem Biol Compound 2Sulfonamide 12bSulfonamide 17Sulfonamide 12aa... |
Ortholog Group (OrthoMCL): OG6_100364
Most Similar Human Ortholog: J3QRN5
TM-align score: 0.96 | RMSD: 0.63
Seq Identity: 0.58 | Length: 65 / 527
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100201 | DEAD-box helicase 17 |
| ENSG00000108654 | DEAD-box helicase 5 |
Protein Length: 527 | Molecular Weight (kDa): 60.036
UniProt ID(s): Q8IL13
PDB ID(s): None
Isoelectric Point: 9.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000629 | ATP-dependent RNA helicase DEAD-box, conserved site |
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 31 | 11 | 30 |
| disruptive | 0 | 8 | 12 | 32 |
| missense | 0 | 8 | 12 | 27 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 40 | 20 | 35 |
| disruptive | 0 | 33 | 33 | 106 |
| missense | 0 | 29 | 26 | 79 |
PlasmoDB Total SNPs: 248
Non-coding: 230 | Synonymous: 16 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 33981864 | Plasmodium falciparum DDX17 is an RNA helicase crucial for parasite development. | Sourabh S, Chauhan M, ..., Tuteja R | 10.1016/j.bbrep.2021.101000 |
| 37774422 | The 3' terminal region of Zika virus RNA contains a conserved G-quadruplex and isunfolded by human DDX17 | Gemmill DL, Nelson CR, ..., Patel TR | 10.1139/bcb-2023-0036 |
| 37239217 | DEAD-Box Helicase 17 Promotes Amyloidogenesis by Regulating BACE1 Translation | Liu Y, Zhou G, ..., Chen G | 10.3390/brainsci13050745 |
| 38303609 | DEAD-Box Helicase 17 exacerbates non-alcoholic steatohepatitis viatranscriptional repression of cyp2c29, inducing hepatic lipid metabolism disorderand eliciting the activation of M1 macrophages | Ning D, Jin J, ..., Zhang W | 10.1002/ctm2.1529 |