Genome location: Pf3D7_14_v3:1,902,569..1,905,593(-)
Genome classification: Core
Product Description: pyruvate dehydrogenase E1 component subunit beta
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 1.2.4.1 (Pyruvate dehydrogenase (acetyl-transferring))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0045254 | pyruvate dehydrogenase complex |
| Function | GO:0004739 | pyruvate dehydrogenase (acetyl-transferring) activity |
| Process | GO:0006086 | acetyl-CoA biosynthetic process from pyruvate |
| Process | GO:0006633 | fatty acid biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | possibly expressed | 0.02 | 0.99 |
| Schizont | possibly expressed | 0.01 | 1.00 |
| Gametocyte | expressed | 0.04 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1446400
Resistome Missense Mutations: M406R
Resistome Compounds with Missense Mutations: BCH070
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.915 | MFS: -2.679 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL09
"Best" AlphaFill ligand hit: MLT ((2r)-2-hydroxybutanedioic acid, Local RMSD=0.03) with 6YAK (Global RMSD=4.65)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.2.4.1 | pyruvate dehydrogenase (acetyl-transferring) | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101309
Most Similar Human Ortholog: P11177
TM-align score: 0.89 | RMSD: 1.28
Seq Identity: 0.35 | Length: 327 / 415
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000168291 | pyruvate dehydrogenase E1 subunit beta |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 1 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 1 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 160
Non-coding: 143 | Synonymous: 10 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 415 | Molecular Weight (kDa): 47.043
PDB ID(s): None
Isoelectric Point: 8.72
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005475 | Transketolase-like, pyrimidine-binding domain |
| InterPro | IPR009014 | Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II |
| InterPro | IPR027110 | Pyruvate dehydrogenase E1 component subunit beta |
| InterPro | IPR029061 | Thiamin diphosphate-binding fold |
| InterPro | IPR033248 | Transketolase, C-terminal domain |
| PFam | PF02779 | Transketolase-like, pyrimidine-binding domain |
| PFam | PF02780 | Transketolase, C-terminal domain |
| Superfamily | SSF52518 | Thiamin diphosphate-binding fold |
| Superfamily | SSF52922 | Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain II |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15612915 | The malaria parasite Plasmodium falciparum has only one pyruvate dehydrogenasecomplex, which is located in the apicoplast. | Foth BJ, Stimmler LM, ..., McFadden GI | 10.1111/j.1365-2958.2004.04407.x |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |