About Gene List

PF3D7_1446700 (AKIT4)

Genome location: Pf3D7_14_v3:1,919,030..1,922,030(-)

Genome classification: Core

Function and Localization

Product Description: apicomplexan kinetochore protein 4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0000776 kinetochore
Component GO:0005634 nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.05 0.97
Trophozoite not expressed 0.32 0.80
Schizont not expressed 0.31 0.78
Gametocyte not expressed 0.42 0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1446700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.49 | MFS: -2.537 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1310500)

  • Relative Growth Rate: 0.02 ± 0.03
  • Confidence: 8.14

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1310500)

Modification: Tagged | RMgm-5116

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A260

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533196

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 27 3 17
disruptive 2 15 11 33
missense 2 15 11 29

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 8 38 14 24
disruptive 5 45 27 67
missense 4 33 25 52

PlasmoDB Total SNPs: 236

Non-coding: 214 | Synonymous: 19 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 357 | Molecular Weight (kDa): 42.631

UniProt ID(s): A0A144A260, Q8IL06

PDB ID(s): None

Isoelectric Point: 4.6

Protein Domain Annotations:

Source Family ID Description
InterPro N/A N/A
PFam N/A N/A
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
36006241 Composition and organization of kinetochores show plasticity in apicomplexanchromosome segregation Brusini L, Dos Santos Pacheco N, ..., Brochet M 10.1083/jcb.202111084