Genome location: Pf3D7_14_v3:1,922,045..1,924,115(+)
Genome classification: Core
Product Description: heme detoxification protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020020 | food vacuole |
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0044164 | host cell cytosol |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0020037 | heme binding |
| Function | GO:0004392 | heme oxygenase (decyclizing) activity |
| Function | GO:0030492 | hemoglobin binding |
| Function | GO:0016829 | lyase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0042167 | heme catabolic process |
| Process | GO:0009405 | obsolete pathogenesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.01 | 1.00 |
| Trophozoite | expressed | 0.03 | 0.98 |
| Schizont | expressed | 0.02 | 0.99 |
| Gametocyte | expressed | 0.06 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1446800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.271 | MFS: -3.645 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1310600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IL04
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=3.33) with 6QV3 (Global RMSD=13.56)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8IL04 | Heme ligase | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | US11365202, Compound 2US11365202, Compound 2US11365202, Compound 3US11365202, Compound 3... |
Protein Length: 205 | Molecular Weight (kDa): 24.337
UniProt ID(s): A0A144A6G1, Q8IL04
PDB ID(s): None
Isoelectric Point: 9.6
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000782 | FAS1 domain |
| InterPro | IPR036378 | FAS1 domain superfamily |
| PFam | PF02469 | FAS1 domain |
| Superfamily | SSF82153 | FAS1 domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 14 | 9 | 14 |
| disruptive | 2 | 9 | 9 | 32 |
| missense | 2 | 9 | 9 | 27 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 26 | 11 | 15 |
| disruptive | 3 | 26 | 24 | 52 |
| missense | 3 | 22 | 20 | 36 |
PlasmoDB Total SNPs: 172
Non-coding: 158 | Synonymous: 10 | Nonsynonymous: 4 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18437218 | HDP-a novel heme detoxification protein from the malaria parasite. | Jani D, Nagarkatti R, ..., Rathore D | 10.1371/journal.ppat.1000053 |
| 23471987 | Protein complex directs hemoglobin-to-hemozoin formation in Plasmodiumfalciparum. | Chugh M, Sundararaman V, ..., Malhotra P | 10.1073/pnas.1218412110 |
| 24025682 | Heme-binding properties of heme detoxification protein from Plasmodiumfalciparum. | Nakatani K, Ishikawa H, Aono S, Mizutani Y | 10.1016/j.bbrc.2013.08.100 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |