PF3D7_1446900

Genome location: Pf3D7_14_v3:1,924,160..1,929,263(-)

Genome classification: Core

Function and Localization

Product Description: glutaminyl-peptide cyclotransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 2.3.2.5 (Glutaminyl-peptide cyclotransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0009986	cell surface

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.19	0.90
Trophozoite	expressed	0.49	0.71
Schizont	expressed	0.33	0.80
Gametocyte	expressed	0.59	0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1446900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.205 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1310700)

Relative Growth Rate: 0.99 ± 0.06
Confidence: 7.08

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1310700)

Modification: Disrupted | RMgm-5096

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A3U7

"Best" AlphaFill ligand hit: DDQ (decylamine-n,n-dimethyl-n-oxide, Local RMSD=0.04) with 3NOK (Global RMSD=3.00)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.2.5	glutaminyl-peptide cyclotransferase	NEMEDTAFADH2ZnCl2NH4ClCuCl2NiCl2PQ912PQ529PBD150nitronSEN177...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_106807

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 195

Non-coding: 180 | Synonymous: 13 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 383 | Molecular Weight (kDa): 44.683

UniProt ID(s): A0A144A3U7

PDB ID(s): None

Isoelectric Point: 8.94

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007788	Glutaminyl-peptide cyclotransferase
InterPro	IPR011044	Quinoprotein amine dehydrogenase, beta chain-like
PFam	PF05096	Glutaminyl-peptide cyclotransferase
Superfamily	SSF50969	Quinoprotein amine dehydrogenase, beta chain-like

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
27128092	Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics.	Swearingen KE, Lindner SE, ..., Sinnis P	10.1371/journal.ppat.1005606