About Gene List

PF3D7_1448400 (HRD3)

Genome location: Pf3D7_14_v3:1,979,973..1,983,139(-)

Genome classification: Core

Function and Localization

Product Description: ubiquitin-protein ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)

Curated GO (PlasmoDB):

Type GO Term Name
Function GO:0008047 enzyme activator activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.10 0.95
Trophozoite expressed 0.73 0.59
Schizont expressed 0.38 0.77
Gametocyte expressed 0.83 0.55

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1448400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.853 | MFS: -2.717 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKY9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
6.3.2.19 ubiquitin-protein ligase No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101047

Most Similar Human Ortholog: A0A2R8Y3T2

TM-align score: 0.84 | RMSD: 3.26

Seq Identity: 0.25 | Length: 381 / 851

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000071537 SEL1L adaptor subunit of ERAD E3 ubiquitin ligase
ENSG00000101251 SEL1L2 adaptor subunit of ERAD E3 ligase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 41 30 72
disruptive 13 61 36 112
missense 13 56 33 88

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 13 82 38 94
disruptive 29 149 69 193
missense 18 125 60 124

PlasmoDB Total SNPs: 81

Non-coding: 43 | Synonymous: 26 | Nonsynonymous: 12 | Stop Codon: 0

Protein Information

Protein Length: 851 | Molecular Weight (kDa): 100.446

UniProt ID(s): Q8IKY9

PDB ID(s): None

Isoelectric Point: 9.19

Protein Domain Annotations:

Source Family ID Description
InterPro IPR006597 Sel1-like repeat
PFam PF08238 Sel1-like repeat
Superfamily SSF81901 N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
26184977 Analysis of antibody production in Saccharomyces cerevisiae: effects of ERprotein quality control disruption de Ruijter JC, Frey AD 10.1007/s00253-015-6807-7