PF3D7_1448800

Genome location: Pf3D7_14_v3:2,001,083..2,002,106(-)

Genome classification: Core

Function and Localization

Product Description: Appr-1-p processing domain protein

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.1.13 (ADP-ribose diphosphatase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	not expressed	0.25	0.84
Schizont	not expressed	0.06	0.96
Gametocyte	expressed	0.28	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1448800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.241 | MFS: -3.2 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1312500)

Relative Growth Rate: 0.12 ± 0.06
Confidence: 6.95

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKY5

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=2.66) with 5L9Q (Global RMSD=5.03)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.1.13	ADP-ribose diphosphatase	ATPADPAMPNMNNADHMgCl2CuSO4MnCl2NaNO2fluoridethymidineADP-ribose...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100645

Most Similar Human Ortholog: A1Z1Q3

TM-align score: 0.65 | RMSD: 2.35

Seq Identity: 0.25 | Length: 194 / 272

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000133315	mono-ADP ribosylhydrolase 1
ENSG00000138496	poly(ADP-ribose) polymerase family member 9
ENSG00000172264	mono-ADP ribosylhydrolase 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	12	13	19
disruptive	4	25	18	32
missense	4	25	18	32

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	25	17	18
disruptive	8	57	34	55
missense	7	46	29	50

PlasmoDB Total SNPs: 79

Non-coding: 61 | Synonymous: 10 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 272 | Molecular Weight (kDa): 31.682

UniProt ID(s): Q8IKY5

PDB ID(s): None

Isoelectric Point: 9.41

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002589	Macro domain
InterPro	IPR043472	Macro domain-like
PFam	PF01661	Macro domain
Superfamily	SSF52949	Macro domain-like