PF3D7_1449300 (BRF1)

Genome location: Pf3D7_14_v3:2,016,601..2,020,055(+)

Genome classification: Core

Function and Localization

Product Description: transcription factor IIIb subunit, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Component	GO:0000126	transcription factor TFIIIB complex
Function	GO:0001006	RNA polymerase III type 3 promoter sequence-specific DNA binding
Function	GO:0008134	transcription factor binding
Process	GO:0006352	DNA-templated transcription, initiation
Process	GO:0070898	RNA polymerase III preinitiation complex assembly

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	nd	nd
Trophozoite	expressed	nd	nd
Schizont	expressed	nd	nd
Gametocyte	not expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1449300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.982 | MFS: -2.543 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A465

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101494

Most Similar Human Ortholog: F8VXJ4

TM-align score: 0.96 | RMSD: 0.56

Seq Identity: 0.54 | Length: 63 / 684

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000185024	BRF1 RNA polymerase III transcription initiation factor subunit

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	40	33	49
disruptive	37	102	65	148
missense	26	87	57	119

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	87	23	60
disruptive	67	202	108	197
missense	43	169	90	131

PlasmoDB Total SNPs: 317

Non-coding: 199 | Synonymous: 64 | Nonsynonymous: 49 | Stop Codon: 5

Protein Information

Protein Length: 684 | Molecular Weight (kDa): 77.94

UniProt ID(s): A0A144A465

PDB ID(s): None

Isoelectric Point: 5.82

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000812	Transcription factor TFIIB
InterPro	IPR011665	Brf1, TBP-binding domain
InterPro	IPR013150	Transcription factor TFIIB, cyclin-like domain
InterPro	IPR013763	Cyclin-like
InterPro	IPR029529	Transcription factor IIIB subunit Brf1
InterPro	IPR036915	Cyclin-like superfamily
PFam	PF00382	Transcription factor TFIIB, cyclin-like domain
PFam	PF07741	Brf1, TBP-binding domain
Superfamily	SSF47954	Cyclin-like superfamily