Genome location: Pf3D7_14_v3:2,019,848..2,024,292(-)
Genome classification: Core
Product Description: crossover junction endonuclease MUS81, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0048476 | Holliday junction resolvase complex |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0048257 | 3'-flap endonuclease activity |
| Function | GO:0004520 | endodeoxyribonuclease activity |
| Process | GO:0006281 | DNA repair |
| Process | GO:0000727 | double-strand break repair via break-induced replication |
| Process | GO:0031573 | mitotic intra-S DNA damage checkpoint signaling |
| Process | GO:0000712 | resolution of meiotic recombination intermediates |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.07 | 0.97 |
| Trophozoite | expressed | 0.40 | 0.76 |
| Schizont | expressed | 0.32 | 0.80 |
| Gametocyte | not expressed | 0.27 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1449400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.13 | MFS: -2.842 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1313100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKY1
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103117
Most Similar Human Ortholog: H0YE94
TM-align score: 0.72 | RMSD: 2.33
Seq Identity: 0.22 | Length: 58 / 1271
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000172732 | MUS81 structure-specific endonuclease subunit |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 81 | 46 | 107 |
| disruptive | 78 | 307 | 145 | 330 |
| missense | 53 | 255 | 126 | 281 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 172 | 68 | 96 |
| disruptive | 158 | 549 | 187 | 420 |
| missense | 105 | 459 | 163 | 283 |
PlasmoDB Total SNPs: 219
Non-coding: 57 | Synonymous: 67 | Nonsynonymous: 95 | Stop Codon: 0
Protein Length: 1271 | Molecular Weight (kDa): 150.049
UniProt ID(s): Q8IKY1
PDB ID(s): None
Isoelectric Point: 8.75
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006166 | ERCC4 domain |
| InterPro | IPR011335 | Restriction endonuclease type II-like |
| InterPro | IPR027421 | DNA polymerase lambda lyase domain superfamily |
| PFam | PF02732 | ERCC4 domain |
| Superfamily | SSF47802 | DNA polymerase lambda lyase domain superfamily |
| Superfamily | SSF52980 | Restriction endonuclease type II-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |