Genome location: Pf3D7_14_v3:2,028,142..2,033,776(+)
Genome classification: Core
Product Description: AP2 domain transcription factor AP2-O5, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000792 | heterochromatin |
| Component | GO:0005634 | nucleus |
| Process | GO:0048870 | cell motility |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.19 | 0.91 |
| Trophozoite | expressed | 0.66 | 0.63 |
| Schizont | expressed | 0.23 | 0.84 |
| Gametocyte | expressed | 0.59 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1449500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.403 | MFS: -2.664 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_1317000)
Modification: Tagged | RMgm-4398
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKY0
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 30 | 19 | 44 |
| disruptive | 31 | 144 | 37 | 152 |
| missense | 20 | 103 | 31 | 111 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 24 | 55 | 36 | 52 |
| disruptive | 71 | 272 | 131 | 291 |
| missense | 38 | 191 | 97 | 146 |
PlasmoDB Total SNPs: 348
Non-coding: 252 | Synonymous: 47 | Nonsynonymous: 45 | Stop Codon: 4
Protein Length: 715 | Molecular Weight (kDa): 84.432
UniProt ID(s): Q8IKY0
PDB ID(s): None
Isoelectric Point: 7.37
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001471 | AP2/ERF domain |
| InterPro | IPR028078 | AP2-coincident, C-terminal domain |
| PFam | PF00847 | AP2/ERF domain |
| PFam | PF14733 | AP2-coincident, C-terminal domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 29233900 | Systematic C RISPR-Cas9-Mediated Modifications of Plasmodium yoelii Api AP2 Genes Reveal Functional Insights into Parasite Development | Zhang C, Li Z, ..., Yuan J | 10.1128/mBio.01986-17 |
| 35288749 | Genome-wide landscape of ApiAP2 transcription factors reveals aheterochromatin-associated regulatory network during Plasmodium falciparumblood-stage development. | Shang X, Wang C, ..., Zhang Q | 10.1093/nar/gkac176 |
| 35871979 | A transcription factor helps Plasmodium falciparum gametocytogenesis take shape | McLean KJ, Niles JC | 10.1016/j.pt.2022.07.004 |
| 35087229 | The transcriptional regulator HDP1 controls expansion of the inner membranecomplex during early sexual differentiation of malaria parasites | Campelo Morillo RA, Tong X, ..., Kafsack BFC | 10.1038/s41564-021-01045-0 |
| 37293082 | PfAP2-MRP DNA-binding protein is a master regulator of parasite pathogenesisduring malaria parasite blood stages | Subudhi AK, Green JL, ..., Pain A | 10.1101/2023.05.23.541898 |
| 37898053 | Cell cycle-regulated ApiAP2s and parasite development: the Toxoplasma paradigm | Zarringhalam K, Ye S, ..., Gubbels MJ | 10.1016/j.mib.2023.102383 |
| 35083215 | PfAP2-EXP2, an Essential Transcription Factor for the IntraerythrocyticDevelopment of Plasmodium falciparum | Shang X, Wang C, ..., Jiang M | 10.3389/fcell.2021.782293 |
| 34400833 | A heat-shock response regulated by the PfAP2-HS transcription factor protectshuman malaria parasites from febrile temperatures | Tinto-Font E, Michel-Todo L, ..., Cortes A | 10.1038/s41564-021-00940-w |
| 38311493 | Sex in a dish: high-efficiency Toxoplasma pre-sexual development | Hryckowian ND, Knoll LJ | 10.1016/j.pt.2024.01.009 |
| 38370614 | Structural characterization of the ACDC domain from ApiAP2 proteins of themalaria parasite | Le Berre M, Tubiana T, ..., Nessler S | 10.1101/2024.02.09.579679 |
| 34874774 | The Extracellular Milieu of Toxoplasma's Lytic Cycle Drives Lab Adaptation,Primarily by Transcriptional Reprogramming | Primo VA Jr, Rezvani Y, ..., Gubbels MJ | 10.1128/mSystems.01196-21 |
| 36292619 | Epigenetic and Epitranscriptomic Gene Regulation in Plasmodium falciparum and HowWe Can Use It against Malaria | Serrano-Duran R, Lopez-Farfan D, Gomez-Diaz E | 10.3390/genes13101734 |
| 34880324 | Essential role of a Plasmodium berghei heat shock protein (PBANKA_0938300) ingametocyte development | Kashif M, Quadiri A, Singh AP | 10.1038/s41598-021-03059-4 |
| 34492224 | Single-genome sequencing reveals within-host evolution of human malariaparasites | Dia A, Jett C, ..., Cheeseman IH | 10.1016/j.chom.2021.08.009 |
| 36223427 | Inhibitors of ApiAP2 protein DNA binding exhibit multistage activity againstPlasmodium parasites | Russell TJ, De Silva EK, ..., Llinas M | 10.1371/journal.ppat.1010887 |
| 36847501 | Single-Cell Transcriptomics To Define Plasmodium falciparum Stage Transition inthe Mosquito Midgut | Mohammed M, Dziedziech A, ..., Ankarklev J | 10.1128/spectrum.03671-22 |
| 37350586 | An apicomplexan bromodomain protein, TgBDP1, associates with diverse epigeneticfactors to regulate essential transcriptional processes in Toxoplasma gondii | Fleck K, McNutt S, Chu F, Jeffers V | 10.1128/mbio.03573-22 |