PF3D7_1450400

Genome location: Pf3D7_14_v3:2,059,507..2,067,011(-)

Genome classification: Core

Function and Localization

Product Description: E3 ubiquitin-protein ligase ZNF598, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.3.2.27 (RING-type E3 ubiquitin transferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.98	0.57
Trophozoite	expressed	1.20	0.41
Schizont	expressed	0.87	0.54
Gametocyte	expressed	0.72	0.62

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1450400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.418 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKX2

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.2.27	RING-type E3 ubiquitin transferase	EDTADTPAMLN4924RAPTA-CRAPTA-TRAPTA-EA1

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101580

Most Similar Human Ortholog: H3BPG6

TM-align score: 0.27 | RMSD: 7.53

Seq Identity: 0.15 | Length: 329 / 1844

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000167962	zinc finger protein 598, E3 ubiquitin ligase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	25	145	79	149
disruptive	62	258	153	354
missense	48	212	136	300

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	59	246	113	169
disruptive	123	605	268	640
missense	82	469	209	472

PlasmoDB Total SNPs: 311

Non-coding: 121 | Synonymous: 106 | Nonsynonymous: 84 | Stop Codon: 0

Protein Information

Protein Length: 1844 | Molecular Weight (kDa): 220.398

UniProt ID(s): Q8IKX2

PDB ID(s): None

Isoelectric Point: 8.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001841	Zinc finger, RING-type
PFam	N/A	N/A
Superfamily	SSF57850	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108