PF3D7_1450600

Genome location: Pf3D7_14_v3:2,075,586..2,077,349(-)

Genome classification: Core

Function and Localization

Product Description: S-adenosylmethionine-dependent methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.12 (Methionine S-methyltransferase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.27	0.87
Trophozoite	possibly expressed	0.35	0.79
Schizont	not expressed	0.05	0.97
Gametocyte	not expressed	0.30	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1450600

Resistome Missense Mutations: S17F, C52Y

Resistome Compounds with Missense Mutations: AN13762, MMV1454442

Resistome # Samples with Disruptive Mutations: 4 (4 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.223 | MFS: -2.648 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1459600)

Relative Growth Rate: 0.06 ± 0.06
Confidence: 6.94

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKX0

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.46) with 2CWW (Global RMSD=2.17)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.12	methionine S-methyltransferase	NEMD-MetGly-MetL-ethionineL-methioninolN-formyl-L-MetL-MethioninamideL-Met ethyl esterL-Met methyl esterseleno-L-methionineL-Met p-nitroanilideS-methyl-L-methionine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104941

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	40	23	45
disruptive	10	78	50	122
missense	10	74	47	113

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	80	29	49
disruptive	28	189	74	180
missense	22	172	67	134

PlasmoDB Total SNPs: 59

Non-coding: 0 | Synonymous: 38 | Nonsynonymous: 20 | Stop Codon: 1

Protein Information

Protein Length: 587 | Molecular Weight (kDa): 68.391

UniProt ID(s): Q8IKX0

PDB ID(s): None

Isoelectric Point: 9.29

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002478	PUA domain
InterPro	IPR019614	S-adenosylmethionine-dependent methyltransferase
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
InterPro	IPR041532	RlmI, PUA-like domain
PFam	PF10672	S-adenosylmethionine-dependent methyltransferase
PFam	PF17785	RlmI, PUA-like domain
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase