PF3D7_1450700

Genome location: Pf3D7_14_v3:2,078,386..2,080,849(+)

Genome classification: Core

Function and Localization

Product Description: Cwf21 domain-containing protein

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.85	0.62
Trophozoite	expressed	0.92	0.52
Schizont	expressed	0.31	0.82
Gametocyte	expressed	0.87	0.54

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1450700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.391 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1314400)

Relative Growth Rate: 0.05 ± 0.11
Confidence: 5.77

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IKW9

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102153

Most Similar Human Ortholog: I3L0N7

TM-align score: 0.50 | RMSD: 3.68

Seq Identity: 0.22 | Length: 78 / 620

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000167978	serine/arginine repetitive matrix 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	43	41	49
disruptive	33	167	86	159
missense	29	154	76	144

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	92	36	51
disruptive	71	306	111	196
missense	50	275	95	136

PlasmoDB Total SNPs: 126

Non-coding: 50 | Synonymous: 34 | Nonsynonymous: 42 | Stop Codon: 0

Protein Information

Protein Length: 620 | Molecular Weight (kDa): 72.758

UniProt ID(s): Q8IKW9

PDB ID(s): None

Isoelectric Point: 10.44

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013170	mRNA splicing factor Cwf21 domain
PFam	PF08312	mRNA splicing factor Cwf21 domain
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108