Genome location: Pf3D7_14_v3:2,089,730..2,094,221(+)
Genome classification: Core
Product Description: elongation factor 2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:1990904 | ribonucleoprotein complex |
| Function | GO:0005525 | GTP binding |
| Function | GO:0003924 | GTPase activity |
| Function | GO:0003723 | RNA binding |
| Function | GO:0043022 | ribosome binding |
| Function | GO:0003746 | translation elongation factor activity |
| Process | GO:0009410 | response to xenobiotic stimulus |
| Process | GO:0006414 | translational elongation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.69 | 0.68 |
| Trophozoite | expressed | 1.09 | 0.45 |
| Schizont | expressed | 0.23 | 0.85 |
| Gametocyte | expressed | 0.44 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1451100
Resistome Missense Mutations: E134D, I183T, Y186N, S474R, A482T, P754A, P754S, L755F
Resistome Compounds with Missense Mutations: DDD107498
Resistome # Samples with Disruptive Mutations: 9 (9 missense, 8 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.368 | MFS: -2.544 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1314800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKW5
"Best" AlphaFill ligand hit: DXC ((3alpha,5beta,12alpha)-3,12-dihydroxycholan-24-oic acid, Local RMSD=0.45) with 4AC9 (Global RMSD=6.59)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100212
Most Similar Human Ortholog: P13639
TM-align score: 0.99 | RMSD: 0.99
Seq Identity: 0.64 | Length: 832 / 832
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000108883 | elongation factor Tu GTP binding domain containing 2 |
| ENSG00000167658 | eukaryotic translation elongation factor 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 33 | 33 | 56 |
| disruptive | 0 | 1 | 3 | 21 |
| missense | 0 | 1 | 3 | 12 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 117 | 20 | 31 |
| disruptive | 1 | 24 | 10 | 49 |
| missense | 0 | 12 | 7 | 28 |
PlasmoDB Total SNPs: 98
Non-coding: 81 | Synonymous: 17 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 832 | Molecular Weight (kDa): 93.523
PDB ID(s): None
Isoelectric Point: 6.77
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000640 | Elongation factor EFG, domain V-like |
| InterPro | IPR000795 | Transcription factor, GTP-binding domain |
| InterPro | IPR004161 | Translation elongation factor EFTu-like, domain 2 |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR005517 | Translation elongation factor EFG/EF2, domain IV |
| InterPro | IPR009000 | Translation protein, beta-barrel domain superfamily |
| InterPro | IPR020568 | Ribosomal protein S5 domain 2-type fold |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031157 | Tr-type G domain, conserved site |
| InterPro | IPR035647 | EF-G domain III/V-like |
| InterPro | IPR041095 | Elongation Factor G, domain II |
| PFam | PF00009 | Transcription factor, GTP-binding domain |
| PFam | PF00679 | Elongation factor EFG, domain V-like |
| PFam | PF03144 | Translation elongation factor EFTu-like, domain 2 |
| PFam | PF03764 | Translation elongation factor EFG/EF2, domain IV |
| PFam | PF14492 | Elongation Factor G, domain II |
| Superfamily | SSF50447 | Translation protein, beta-barrel domain superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF54211 | Ribosomal protein S5 domain 2-type fold |
| Superfamily | SSF54980 | EF-G domain III/V-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 26085270 | A novel multiple-stage antimalarial agent that inhibits protein synthesis. | Baragana B, Hallyburton I, ..., Gilbert IH | 10.1038/nature14451 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 38097134 | Inositol hexaphosphate enhances chemotherapy by reversing senescence induced bypersistently activated PERK and diphthamide modification of eEF2 | Xu B, Jia Q, ..., Liang L | 10.1016/j.canlet.2023.216591 |
| 38395592 | Cryo-EM structure of the inactive ribosome complex accumulated in chick embryocells in cold-stress conditions | Nurullina L, Terrosu S, ..., Yusupov M | 10.1002/1873-3468.14831 |
| 38106175 | Translation inhibition efficacy does not determine the Plasmodium berghei liverstage antiplasmodial efficacy of protein synthesis inhibitors | McLellan JL, Hanson KK | 10.1101/2023.12.07.570699 |
| 38103971 | Revealing eEF-2 kinase: recent structural insights into function | Piserchio A, Dalby KN, Ghose R | 10.1016/j.tibs.2023.11.004 |
| 38030725 | mRNA reading frame maintenance during eukaryotic ribosome translocation | Milicevic N, Jenner L, ..., Yusupova G | 10.1038/s41586-023-06780-4 |
| 38219816 | Ribosome inactivation regulates translation elongation in neurons | Popper B, Burkle M, ..., Schieweck R | 10.1016/j.jbc.2024.105648 |
| 38097404 | Diphthamide - a conserved modification of eEF2 with clinical relevance | Schaffrath R, Brinkmann U | 10.1016/j.molmed.2023.11.008 |