Genome location: Pf3D7_14_v3:2,104,693..2,107,407(+)
Genome classification: Core
Product Description: E3 SUMO-protein ligase NSE2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.2.27 (RING-type E3 ubiquitin transferase);6.3.2.- (Acid--amino-acid ligases (peptide synthases).)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0030915 | Smc5-Smc6 complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0061665 | SUMO ligase activity |
| Process | GO:0000724 | double-strand break repair via homologous recombination |
| Process | GO:0016925 | protein sumoylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.07 | 0.97 |
| Trophozoite | expressed | 0.34 | 0.78 |
| Schizont | possibly expressed | 0.32 | 0.79 |
| Gametocyte | possibly expressed | 0.44 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1451300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.624 | MFS: -2.425 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKW3
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 16 | 5 | 17 |
| disruptive | 0 | 13 | 15 | 31 |
| missense | 0 | 13 | 14 | 25 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 28 | 11 | 19 |
| disruptive | 4 | 47 | 27 | 63 |
| missense | 3 | 40 | 23 | 39 |
PlasmoDB Total SNPs: 98
Non-coding: 88 | Synonymous: 6 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 229 | Molecular Weight (kDa): 27.608
UniProt ID(s): Q8IKW3
PDB ID(s): None
Isoelectric Point: 6.11
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004181 | Zinc finger, MIZ-type |
| InterPro | IPR026846 | E3 SUMO-protein ligase Nse2 (Mms21) |
| PFam | PF11789 | Zinc finger, MIZ-type |
| Superfamily | SSF57850 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34853311 | Structural basis for the E3 ligase activity enhancement of yeast Nse2 by SUMO-interacting motifs | Varejao N, Lascorz J, ..., Reverter D | 10.1038/s41467-021-27301-9 |
| 29794112 | How DNA vicinity controls SUMO E3 ligase activity | Pichler A | 10.15252/embj.201899705 |
| 34695110 | The SUMO ligase MMS21 profoundly influences maize development through its impacton genome activity and stability | Zhang J, Augustine RC, ..., Vierstra RD | 10.1371/journal.pgen.1009830 |
| 27215325 | Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components | Bustard DE, Ball LG, Cobb JA | 10.1242/bio.018440 |
| 27872982 | Interplay between Top1 and Mms21/Nse2 mediated sumoylation in stable maintenanceof long chromosomes | Mahendrawada L, Rai R, Kothiwal D, Laloraya S | 10.1007/s00294-016-0665-4 |
| 33941673 | Integrative analysis reveals unique structural and functional features of the Smc5/6 complex | Yu Y, Li S, ..., Zhao X | 10.1073/pnas.2026844118 |
| 29769404 | DNA activates the Nse2/Mms21 SUMO E3 ligase in the Smc5/6 complex | Varejao N, Ibars E, ..., Reverter D | 10.15252/embj.201798306 |
| 32964921 | Smc5/6, an atypical SMC complex with two RING-type subunits | Sole-Soler R, Torres-Rosell J | 10.1042/BST20200389 |
| 35612306 | Smc5/6 Complex Promotes Rad3(ATR) Checkpoint Signaling at the Perturbed Replication Fork through Sumoylation of the RecQ Helicase Rqh1 | Khan S, Ahamad N, ..., Xu YJ | 10.1128/mcb.00045-22 |
| 31801080 | Sumoylation of Smc5 Promotes Error-free Bypass at Damaged Replication Forks | Zapatka M, Pocino-Merino I, ..., Torres-Rosell J | 10.1016/j.celrep.2019.10.123 |