Genome location: Pf3D7_14_v3:2,115,436..2,118,190(-)
Genome classification: Core
Product Description: LCCL domain-containing protein
SignalP Peptide: MKYINTICFVLIFLSSKCNT
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0044312 | crystalloid |
| Function | GO:0044877 | protein-containing complex binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | not expressed | 0.03 | 0.98 |
| Schizont | not expressed | 0.12 | 0.92 |
| Gametocyte | expressed | 0.70 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1451600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.366 | MFS: -2.734 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1315300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1315300)
Modification: Disrupted | RMgm-3480
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKW0
"Best" AlphaFill ligand hit: GIM (glucoimidazole, Local RMSD=1.90) with 5BX4 (Global RMSD=17.00)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P13423 | Protective antigen | Bacillus anthracis | OrthoMCL BLAST | CHEMBL2179142CHEMBL2179141CHEMBL2179142CHEMBL2179141... |
Protein Length: 865 | Molecular Weight (kDa): 100.048
UniProt ID(s): Q8IKW0
PDB ID(s): None
Isoelectric Point: 8.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011658 | PA14 domain |
| InterPro | IPR039269 | Ankyrin repeat and fibronectin type-III domain-containing protein 1 |
| PFam | PF07691 | PA14 domain |
| Superfamily | SSF56988 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 62 | 37 | 58 |
| disruptive | 7 | 91 | 58 | 123 |
| missense | 7 | 91 | 53 | 113 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 19 | 122 | 31 | 67 |
| disruptive | 20 | 215 | 87 | 192 |
| missense | 15 | 193 | 78 | 146 |
PlasmoDB Total SNPs: 52
Non-coding: 8 | Synonymous: 30 | Nonsynonymous: 14 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 19304662 | Sexual stage adhesion proteins form multi-protein complexes in the malariaparasite Plasmodium falciparum. | Simon N, Scholz SM, ..., Pradel G | 10.1074/jbc.M808472200 |
| 23684590 | Translational repression controls temporal expression of the Plasmodium bergheiLCCL protein complex. | Saeed S, Carter V, Tremp AZ, Dessens JT | 10.1016/j.molbiopara.2013.04.006 |
| 32183975 | Live-cell imaging of mitochondrial motility and interactions in Drosophilaneurons and yeast | Liao PC, Higuchi-Sanabria R, ..., Pon LA | 10.1016/bs.mcb.2019.11.011 |
| 35928001 | Enrichment of aging yeast cells and budding polarity assay in Saccharomycescerevisiae | Yang EJ, Pon LA | 10.1016/j.xpro.2022.101599 |
| 34542849 | Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells | Sing CN, Yang EJ, ..., Swayne TC | 10.1007/978-1-0716-1661-1_4 |
| 35577788 | Identification of a modulator of the actin cytoskeleton, mitochondria, nutrientmetabolism and lifespan in yeast | Sing CN, Garcia EJ, ..., Pon LA | 10.1038/s41467-022-30045-9 |
| 28414172 | LCCL protein complex formation in Plasmodium is critically dependent on LAP1 | Tremp AZ, Sharma V, ..., Dessens JT | 10.1016/j.molbiopara.2017.04.005 |
| 32183963 | Live cell imaging of mitochondrial redox state in mammalian cells and yeast | Liao PC, Franco-Iborra S, Yang Y, Pon LA | 10.1016/bs.mcb.2019.11.008 |
| 26764088 | Mitochondrial anchorage and fusion contribute to mitochondrial inheritance andquality control in the budding yeast Saccharomyces cerevisiae | Higuchi-Sanabria R, Charalel JK, ..., Pon LA | 10.1091/mbc.E15-07-0455 |
| 34542848 | Imaging the Actin Cytoskeleton in Live Budding Yeast Cells | Sing CN, Yang EJ, ..., Pon LA | 10.1007/978-1-0716-1661-1_3 |