Genome location: Pf3D7_14_v3:2,133,266..2,141,180(+)
Genome classification: Core
Product Description: rhoptry neck protein 2
SignalP Peptide: MLKFFIFILHIYLYIDSIYS
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0032991 | protein-containing complex |
| Component | GO:1990225 | rhoptry neck |
| Function | GO:0005515 | protein binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.12 | 0.93 |
| Schizont | expressed | 2.19 | 0.25 |
| Gametocyte | not expressed | 0.25 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1452000
Resistome Missense Mutations: G462E, T463S, S466P, E471G
Resistome Compounds with Missense Mutations: Cipargamin/KAE609/NITD609, Proguanil
Resistome # Samples with Disruptive Mutations: 9 (9 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.229 | MFS: -2.619 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1315700)
Modification: Mutated | RMgm-5151
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKV6
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 70 | 55 | 108 |
| disruptive | 129 | 203 | 114 | 284 |
| missense | 76 | 144 | 91 | 209 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 37 | 132 | 75 | 127 |
| disruptive | 175 | 405 | 189 | 353 |
| missense | 103 | 286 | 135 | 206 |
PlasmoDB Total SNPs: 361
Non-coding: 106 | Synonymous: 117 | Nonsynonymous: 138 | Stop Codon: 0
Protein Length: 2189 | Molecular Weight (kDa): 249.487
UniProt ID(s): B9A597, B9A598, B9A599, B9A5A0, Q8IKV6
PDB ID(s): 3SRI, 3ZWZ, 4Z09, 4Z0D, 4Z0E, 4Z0F, 8GID, 8GIE, 8GIF
Isoelectric Point: 9.07
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368868 | Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14. | Gardner MJ, Shallom SJ, ..., Fraser CM | 10.1038/nature01094 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 18952195 | Rhoptry neck protein RON2 forms a complex with microneme protein AMA1 inPlasmodium falciparum merozoites. | Cao J, Kaneko O, ..., Torii M | 10.1016/j.parint.2008.09.005 |
| 22737069 | Structural and functional insights into the malaria parasite moving junctioncomplex. | Vulliez-Le Normand B, Tonkin ML, ..., Lebrun M | 10.1371/journal.ppat.1002755 |
| 24958881 | Immunization with a functional protein complex required for erythrocyte invasionprotects against lethal malaria. | Srinivasan P, Ekanem E, ..., Miller LH | 10.1073/pnas.1409928111 |
| 26731670 | Stability of the Plasmodium falciparum AMA1-RON2 Complex Is Governed by theDomain II (DII) Loop. | Delgadillo RF, Parker ML, ..., Douguet D | 10.1371/journal.pone.0144764 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 32817376 | Detection of the Rhoptry Neck Protein Complex in Plasmodium Sporozoites and Its Contribution to Sporozoite Invasion of Salivary Glands | Nozaki M, Baba M, ..., Ishino T | 10.1128/mSphere.00325-20 |
| 36577076 | The direct binding of Plasmodium vivax AMA1 to erythrocytes defines aRON2-independent invasion pathway | Lee SK, Low LM, ..., Gunalan K | 10.1073/pnas.2215003120 |
| 36847896 | Defining species-specific and conserved interactions of apical membrane protein 1during erythrocyte invasion in malaria to inform multi-species vaccines | Drew DR, Wilson DW, ..., Beeson JG | 10.1007/s00018-023-04712-z |
| 37660103 | Structure-based design of a strain transcending AMA1-RON2L malaria vaccine | Patel PN, Dickey TH, ..., Tolia NH | 10.1038/s41467-023-40878-7 |
| 37272704 | Rhoptry neck protein 4 plays important roles during Plasmodium sporozoiteinfection of the mammalian liver | Baba M, Nozaki M, ..., Ishino T | 10.1128/msphere.00587-22 |
| 36512904 | Proteomic characterization of Toxoplasma gondii ME49 derived strains resistant tothe artemisinin derivatives artemiside and artemisone implies potential mode ofaction independent of ROS formation | Muller J, Schlange C, ..., Hemphill A | 10.1016/j.ijpddr.2022.11.005 |
| 37131813 | Structure guided mimicry of an essential P. falciparum receptor-ligand complexenhances cross neutralizing antibodies | Srinivasan P, Yanik S, ..., Boulanger M | 10.21203/rs.3.rs-2733434/v1 |
| 35731833 | The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito andmammalian hosts | Fernandes P, Loubens M, ..., Silvie O | 10.1371/journal.ppat.1010643 |
| 34390881 | cAMP-dependent protein kinase regulates secretion of apical membrane antigen 1(AMA1) in Plasmodium yoelii | Ishizaki T, Asada M, ..., Kaneko O | 10.1016/j.parint.2021.102435 |
| 38370683 | Potent AMA1-specific human monoclonal antibody against P. vivax Pre-erythrocyticand Blood Stages | Winnicki AC, Dietrich MH, ..., King CL | 10.1101/2024.02.07.579302 |
| 33430886 | Isolation and light chain shuffling of a Plasmodium falciparum AMA1-specifichuman monoclonal antibody with growth inhibitory activity | Seidel-Greven M, Addai-Mensah O, ..., Fendel R | 10.1186/s12936-020-03548-3 |
| 36504817 | Vesicular stomatitis virus-based vaccine targeting plasmodium blood-stageantigens elicits immune response and protects against malaria with proteinbooster strategy | Sun Y, Shi X, ..., Cheng Y | 10.3389/fmicb.2022.1042414 |
| 36693095 | Invasion of Toxoplasma gondii bradyzoites: Molecular dissection of the movingjunction proteins and effective vaccination targets | Najm R, Ruivo MTG, ..., Lebrun M | 10.1073/pnas.2219533120 |
| 37735574 | Structure guided mimicry of an essential P. falciparum receptor-ligand complexenhances cross neutralizing antibodies | Yanik S, Venkatesh V, ..., Srinivasan P | 10.1038/s41467-023-41636-5 |