Genome location: Pf3D7_14_v3:2,228,509..2,231,910(+)
Genome classification: Core
Product Description: SNF1-related serine/threonine protein kinase KIN
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0009594 | detection of nutrient |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.13 | 0.94 |
| Trophozoite | not expressed | 0.09 | 0.94 |
| Schizont | possibly expressed | 0.05 | 0.97 |
| Gametocyte | expressed | 0.39 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1454300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.692 | #Insertions: 4
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1318000)
Modification: Mutated | RMgm-4192
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKT6
"Best" AlphaFill ligand hit: BUD ((2s,3s)-butane-2,3-diol, Local RMSD=0.06) with 3OVV (Global RMSD=1.84)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 37 | 38 | 54 |
| disruptive | 15 | 88 | 57 | 131 |
| missense | 14 | 87 | 56 | 123 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 19 | 92 | 37 | 66 |
| disruptive | 32 | 233 | 110 | 260 |
| missense | 27 | 203 | 90 | 181 |
PlasmoDB Total SNPs: 192
Non-coding: 121 | Synonymous: 43 | Nonsynonymous: 27 | Stop Codon: 1
Protein Length: 765 | Molecular Weight (kDa): 90.855
PDB ID(s): None
Isoelectric Point: 10.22
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 28678779 | Nutrient sensing modulates malaria parasite virulence. | Mancio-Silva L, Slavic K, ..., Mota MM | 10.1038/nature23009 |