Genome location: Pf3D7_14_v3:2,232,798..2,236,926(+)
Genome classification: Core
Product Description: aminopeptidase P
SignalP Peptide: MQLNFLLFVFIFLMVFH
# Transmembrane Domains: 1
EC Numbers: 3.4.11.9 (Xaa-Pro aminopeptidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0020020 | food vacuole |
| Function | GO:0004177 | aminopeptidase activity |
| Function | GO:0070006 | metalloaminopeptidase activity |
| Function | GO:0042803 | protein homodimerization activity |
| Process | GO:0042540 | hemoglobin catabolic process |
| Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.85 | 0.61 |
| Trophozoite | expressed | 1.45 | 0.33 |
| Schizont | expressed | 0.30 | 0.81 |
| Gametocyte | expressed | 0.65 | 0.64 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1454400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 6 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.142 | MFS: -3.25 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1318100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1318100)
Modification: Disrupted | RMgm-813
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A2H0
"Best" AlphaFill ligand hit: AR6 (adenosine-5-diphosphoribose, Local RMSD=14.38) with 8DMR (Global RMSD=18.41)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.11.9 | Xaa-Pro aminopeptidase | NEMDTTEDTAPCMBEGTAPMSFLeu-ProArg-ProSer-ProCH3HgClbestatinantipain... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100896
Most Similar Human Ortholog: Q5T6H7
TM-align score: 0.93 | RMSD: 1.88
Seq Identity: 0.33 | Length: 533 / 777
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000108039 | X-prolyl aminopeptidase 1 |
| ENSG00000122121 | X-prolyl aminopeptidase 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 41 | 27 | 58 |
| disruptive | 29 | 63 | 40 | 113 |
| missense | 24 | 59 | 37 | 90 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 90 | 33 | 63 |
| disruptive | 39 | 177 | 108 | 219 |
| missense | 29 | 143 | 84 | 120 |
PlasmoDB Total SNPs: 227
Non-coding: 165 | Synonymous: 40 | Nonsynonymous: 22 | Stop Codon: 0
Protein Length: 777 | Molecular Weight (kDa): 90.13
UniProt ID(s): A0A144A2H0
Isoelectric Point: 6.79
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000587 | Creatinase, N-terminal |
| InterPro | IPR000994 | Peptidase M24 |
| InterPro | IPR001131 | Peptidase M24B, X-Pro dipeptidase/aminopeptidase P, conserved site |
| InterPro | IPR029149 | Creatinase/Aminopeptidase P/Spt16, N-terminal |
| InterPro | IPR032416 | Peptidase M24, C-terminal domain |
| InterPro | IPR033740 | Aminopeptidase P |
| InterPro | IPR036005 | Creatinase/aminopeptidase-like |
| PFam | PF00557 | Peptidase M24 |
| PFam | PF01321 | Creatinase, N-terminal |
| PFam | PF16188 | Peptidase M24, C-terminal domain |
| PFam | PF16189 | |
| Superfamily | SSF53092 | Creatinase/Aminopeptidase P/Spt16, N-terminal |
| Superfamily | SSF55920 | Creatinase/aminopeptidase-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19574214 | Evidence for catalytic roles for Plasmodium falciparum aminopeptidase P in thefood vacuole and cytosol. | Ragheb D, Bompiani K, Dalal S, Klemba M | 10.1074/jbc.M109.018424 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 27458998 | Transcripts in the Plasmodium Apicoplast Undergo Cleavage at tRNAs and Editing,and Include Antisense Sequences. | Nisbet RER, Kurniawan DP, Bowers HD, Howe CJ | 10.1016/j.protis.2016.06.003 |