Genome location: Pf3D7_14_v3:2,237,754..2,239,406(+)
Genome classification: Core
Product Description: iron-sulfur cluster assembly protein ISCU
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005759 | mitochondrial matrix |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0051537 | 2 iron, 2 sulfur cluster binding |
| Function | GO:0051539 | 4 iron, 4 sulfur cluster binding |
| Function | GO:0008198 | ferrous iron binding |
| Function | GO:0005506 | iron ion binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0006879 | cellular iron ion homeostasis |
| Process | GO:0016226 | iron-sulfur cluster assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.23 | 0.88 |
| Trophozoite | expressed | 0.14 | 0.91 |
| Schizont | expressed | 0.09 | 0.94 |
| Gametocyte | expressed | 0.23 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1454500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.141 | MFS: -3.156 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1318200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKT4
"Best" AlphaFill ligand hit: 15P (peg 1500, Local RMSD=0.86) with 3IAU (Global RMSD=17.05)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100973
Most Similar Human Ortholog: Q9H1K1
TM-align score: 0.79 | RMSD: 1.50
Seq Identity: 0.57 | Length: 134 / 162
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000136003 | iron-sulfur cluster assembly enzyme |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 51
Non-coding: 42 | Synonymous: 7 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 162 | Molecular Weight (kDa): 18.057
UniProt ID(s): Q8IKT4
PDB ID(s): None
Isoelectric Point: 9.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002871 | NIF system FeS cluster assembly, NifU, N-terminal |
| InterPro | IPR011339 | ISC system FeS cluster assembly, IscU scaffold |
| PFam | PF01592 | NIF system FeS cluster assembly, NifU, N-terminal |
| Superfamily | SSF82649 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34032321 | [Fe-S] biogenesis and unusual assembly of the ISC scaffold complex in thePlasmodium falciparum mitochondrion. | Mohammad Sadik, Mohammad Afsar, Ramachandran R, Habib S | 10.1111/mmi.14735 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |