Genome location: Pf3D7_14_v3:2,240,977..2,243,543(+)
Genome classification: Core
Product Description: 6-phosphogluconate dehydrogenase, decarboxylating
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.1.1.44 (Phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005622 | intracellular |
| Component | GO:0005634 | nucleus |
| Function | GO:0050661 | NADP binding |
| Function | GO:0004616 | phosphogluconate dehydrogenase (decarboxylating) activity |
| Process | GO:0046177 | D-gluconate catabolic process |
| Process | GO:0006098 | pentose-phosphate shunt |
| Process | GO:0009051 | pentose-phosphate shunt, oxidative branch |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.32 | 0.84 |
| Trophozoite | expressed | 0.83 | 0.55 |
| Schizont | expressed | 0.21 | 0.86 |
| Gametocyte | expressed | 0.37 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1454700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.063 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1318400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_1322200)
Modification: Tagged | RMgm-1572
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKT2
"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.01) with 8I4Q (Global RMSD=1.67)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.1.1.44 | phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) | ATPADPUDPCO2AMPGTPGDPCTPUTPCMPUMPGMP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P52209 | 6-phosphogluconate dehydrogenase, decarboxylating | Homo sapiens | HOGENOM, OMA, OrthoDB | Sulfonamide derivative, 5KETOTIFENCHEMBL323922CHEMBL116018... |
| Q38DI7 | 6-phosphogluconate dehydrogenase, decarboxylating | Trypanosoma brucei brucei (strain 927/4 GUTat10.1) | OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | CHEMBL1235136CHEMBL324609CHEMBL114269CHEMBL116018... |
| P00349 | 6-phosphogluconate dehydrogenase, decarboxylating | Ovis aries | OrthoDB | CHEMBL324609CHEMBL114269CHEMBL116018CHEMBL114269... |
| P85968 | 6-phosphogluconate dehydrogenase, decarboxylating | OrthoDB | CHEMBL5174714CHEMBL5174714 |
Ortholog Group (OrthoMCL): OG6_100975
Most Similar Human Ortholog: P52209
TM-align score: 0.97 | RMSD: 1.20
Seq Identity: 0.44 | Length: 465 / 468
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000142657 | phosphogluconate dehydrogenase |
Protein Length: 468 | Molecular Weight (kDa): 52.994
UniProt ID(s): Q8IKT2
Isoelectric Point: 6.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006113 | 6-phosphogluconate dehydrogenase, decarboxylating |
| InterPro | IPR006114 | 6-phosphogluconate dehydrogenase, C-terminal |
| InterPro | IPR006115 | 6-phosphogluconate dehydrogenase, NADP-binding |
| InterPro | IPR006183 | 6-phosphogluconate dehydrogenase |
| InterPro | IPR008927 | 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF00393 | 6-phosphogluconate dehydrogenase, C-terminal |
| PFam | PF03446 | 6-phosphogluconate dehydrogenase, NADP-binding |
| Superfamily | SSF48179 | 6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 23 | 9 | 25 |
| disruptive | 2 | 17 | 16 | 27 |
| missense | 2 | 17 | 15 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 42 | 21 | 24 |
| disruptive | 4 | 43 | 22 | 70 |
| missense | 3 | 41 | 19 | 44 |
PlasmoDB Total SNPs: 104
Non-coding: 80 | Synonymous: 19 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27503796 | Proteome mapping of Plasmodium: identification of the P. yoelii remodellome. | Siau A, Huang X, ..., Preiser PR | 10.1038/srep31055 |
| 30098336 | Characterization of Plasmodium falciparum 6-Phosphogluconate Dehydrogenase as anAntimalarial Drug Target. | Haeussler K, Fritz-Wolf K, ..., Becker K | 10.1016/j.jmb.2018.07.030 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 37599457 | Structural and functional characterization of 6-phosphogluconate dehydrogenase inPlasmodium falciparum (3D7) and identification of its potent inhibitors | Karthika A, Hemavathy N, ..., Jeyakanthan J | 10.1080/07391102.2023.2248271 |