Genome location: Pf3D7_14_v3:2,250,677..2,251,978(-)
Genome classification: Core
Product Description: protein tyrosine phosphatase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.48 (Protein-tyrosine-phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0045177 | apical part of cell |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0017017 | MAP kinase tyrosine/serine/threonine phosphatase activity |
| Function | GO:0051019 | mitogen-activated protein kinase binding |
| Function | GO:0004721 | phosphoprotein phosphatase activity |
| Function | GO:0008330 | protein tyrosine/threonine phosphatase activity |
| Process | GO:0000188 | obsolete inactivation of MAPK activity |
| Process | GO:0035970 | peptidyl-threonine dephosphorylation |
| Process | GO:0035335 | peptidyl-tyrosine dephosphorylation |
| Process | GO:0006470 | protein dephosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.99 | 0.58 |
| Trophozoite | expressed | 0.55 | 0.68 |
| Schizont | not expressed | 0.20 | 0.87 |
| Gametocyte | not expressed | 0.70 | 0.62 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1455100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.831 | MFS: -0.409 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1318800)
Modification: Tagged | RMgm-1083
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKS8
"Best" AlphaFill ligand hit: 4NP (4-nitrophenyl phosphate, Local RMSD=0.33) with 4WOH (Global RMSD=8.13)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.48 | protein-tyrosine-phosphatase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9NRW4 | Dual specificity protein phosphatase 22 | Homo sapiens | OMA | CHEMBL183967CHEMBL365871CHEMBL435096CHEMBL183967... |
Ortholog Group (OrthoMCL): OG6_107960
Most Similar Human Ortholog: Q16829
TM-align score: 0.80 | RMSD: 1.73
Seq Identity: 0.37 | Length: 145 / 171
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000164086 | dual specificity phosphatase 7 |
Protein Length: 171 | Molecular Weight (kDa): 19.873
UniProt ID(s): Q8IKS8
PDB ID(s): None
Isoelectric Point: 6.44
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000340 | Dual specificity phosphatase, catalytic domain |
| InterPro | IPR020422 | Dual specificity protein phosphatase domain |
| InterPro | IPR029021 | Protein-tyrosine phosphatase-like |
| PFam | PF00782 | Dual specificity phosphatase, catalytic domain |
| Superfamily | SSF52799 | Protein-tyrosine phosphatase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 9 | 2 | 12 |
| disruptive | 1 | 5 | 2 | 12 |
| missense | 1 | 5 | 2 | 12 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 18 | 6 | 14 |
| disruptive | 3 | 20 | 9 | 36 |
| missense | 1 | 14 | 9 | 28 |
PlasmoDB Total SNPs: 94
Non-coding: 85 | Synonymous: 8 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 31289175 | Positional Dynamics and Glycosomal Recruitment of Developmental Regulators duringTrypanosome Differentiation | Szoor B, Simon DV, ..., Matthews KR | 10.1128/mBio.00875-19 |
| 30417267 | Yeast two-hybrid screening identifies MPZ-1 and PTP-1 as candidate scaffoldingproteins of metabotropic glutamate receptors in Caenorhabditis elegans | Dillon J, Holden-Dye L, O'Connor V | 10.1007/s10158-018-0218-2 |
| 30036623 | Chemical characterization and antioxidant activities of polysaccharides isolatedfrom the stems of Parthenocissus tricuspidata | Liang X, Gao Y, ..., Zhang W | 10.1016/j.ijbiomac.2018.07.131 |