PF3D7_1455200

Genome location: Pf3D7_14_v3:2,253,099..2,257,505(+)

Genome classification: Core

Function and Localization

Product Description: methyltransferase AAMT

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.201 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0045177	apical part of cell

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	nd	nd
Trophozoite	expressed	nd	nd
Schizont	expressed	nd	nd
Gametocyte	expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1455200.2

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.763 | MFS: -2.051 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1006900)

Relative Growth Rate: 1.03 ± 0.13
Confidence: 5.51

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A144A2R4

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.61) with 2PXX (Global RMSD=1.65)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.201	2-methoxy-6-polyprenyl-1,4-benzoquinol methylase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100611

Most Similar Human Ortholog: Q8N6R0

TM-align score: 0.89 | RMSD: 2.04

Seq Identity: 0.30 | Length: 199 / 205

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000010165	eEF1A lysine and N-terminal methyltransferase
ENSG00000284753	EEF1A lysine methyltransferase 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 219

Non-coding: 206 | Synonymous: 9 | Nonsynonymous: 3 | Stop Codon: 1

Protein Information

Protein Length: 205 | Molecular Weight (kDa): 23.99

UniProt ID(s): A0A144A2R4

PDB ID(s): None

Isoelectric Point: 4.97

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013216	Methyltransferase type 11
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF08241	Methyltransferase type 11
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase