Genome location: Pf3D7_14_v3:2,285,113..2,290,227(+)
Genome classification: Core
Product Description: LCCL domain-containing protein
SignalP Peptide: MTKLFFINYAFVIIFSFNLFVKC
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0044312 | crystalloid |
| Component | GO:0032991 | protein-containing complex |
| Component | GO:0020003 | symbiont-containing vacuole |
| Process | GO:0051301 | cell division |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | not expressed | 0.04 | 0.97 |
| Schizont | not expressed | 0.02 | 0.98 |
| Gametocyte | expressed | 0.61 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1455800
Resistome Missense Mutations: I14S
Resistome Compounds with Missense Mutations: MMV011895
Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.979 | MFS: -2.053 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1319500)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_1323300)
Modification: Tagged | RMgm-5294
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKS0
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 97 | 42 | 118 |
| disruptive | 11 | 106 | 72 | 193 |
| missense | 10 | 103 | 71 | 171 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 27 | 183 | 82 | 105 |
| disruptive | 28 | 241 | 135 | 308 |
| missense | 26 | 207 | 122 | 247 |
PlasmoDB Total SNPs: 117
Non-coding: 34 | Synonymous: 60 | Nonsynonymous: 21 | Stop Codon: 2
Protein Length: 1617 | Molecular Weight (kDa): 184.696
UniProt ID(s): Q8IKS0
PDB ID(s): None
Isoelectric Point: 4.85
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000421 | Coagulation factor 5/8 C-terminal domain |
| InterPro | IPR000772 | Ricin B, lectin domain |
| InterPro | IPR004043 | LCCL domain |
| InterPro | IPR008979 | Galactose-binding-like domain superfamily |
| InterPro | IPR035992 | Ricin B-like lectins |
| InterPro | IPR036056 | Fibrinogen-like, C-terminal |
| InterPro | IPR036609 | LCCL domain superfamily |
| PFam | PF00754 | Coagulation factor 5/8 C-terminal domain |
| PFam | PF03815 | LCCL domain |
| Superfamily | SSF49785 | Galactose-binding-like domain superfamily |
| Superfamily | SSF50370 | Ricin B-like lectins |
| Superfamily | SSF56496 | Fibrinogen-like, C-terminal |
| Superfamily | SSF69848 | LCCL domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15184503 | A multidomain adhesion protein family expressed in Plasmodium falciparum isessential for transmission to the mosquito. | Pradel G, Hayton K, ..., Templeton TJ | 10.1084/jem.20031274 |
| 19304662 | Sexual stage adhesion proteins form multi-protein complexes in the malariaparasite Plasmodium falciparum. | Simon N, Scholz SM, ..., Pradel G | 10.1074/jbc.M808472200 |
| 23684590 | Translational repression controls temporal expression of the Plasmodium bergheiLCCL protein complex. | Saeed S, Carter V, Tremp AZ, Dessens JT | 10.1016/j.molbiopara.2013.04.006 |
| 30367865 | The Plasmodium LAP complex affects crystalloid biogenesis and oocyst celldivision. | Saeed S, Tremp AZ, Dessens JT | 10.1016/j.ijpara.2018.09.002 |
| 36424922 | Extraction and immunomodulatory activity of the polysaccharide obtained fromCraterellus cornucopioides | Zhang C, Shu Y, Li Y, Guo M | 10.3389/fnut.2022.1017431 |