Genome location: Pf3D7_14_v3:2,290,465..2,293,029(-)
Genome classification: Core
Product Description: polyprenol reductase
SignalP Peptide: N/A
# Transmembrane Domains: 7
EC Numbers: 1.3.1.94 (Polyprenol reductase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0003865 | 3-oxo-5-alpha-steroid 4-dehydrogenase activity |
| Function | GO:0102389 | polyprenol reductase activity |
| Process | GO:0019408 | dolichol biosynthetic process |
| Process | GO:0019348 | dolichol metabolic process |
| Process | GO:0006488 | dolichol-linked oligosaccharide biosynthetic process |
| Process | GO:0016095 | polyprenol catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.08 | 0.96 |
| Trophozoite | no data | 0.44 | 0.74 |
| Schizont | no data | 0.17 | 0.89 |
| Gametocyte | no data | 0.21 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1455900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.152 | MFS: -3.025 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1319600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6S3J5
"Best" AlphaFill ligand hit: NDP (nadph dihydro-nicotinamide-adenine-dinucleotide phosphate, Local RMSD=1.15) with 7C83 (Global RMSD=1.40)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.3.1.94 | polyprenol reductase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P18405 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 | OrthoDB | 17-Oxaandrost-5-ene-3β-yl-benzoate (1)17-Oxaandrost-5-ene-3β-yl-p-fluorobenzoate (2)17-Oxaandrost-5-ene-3β-yl-p-chlorobenzoate (3)17-Oxaandrost-5-ene-3β-yl-p-bromobenzoate (4)... | |
| P24008 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 | Rattus norvegicus | OrthoDB | EpristerideCHEMBL79036CHEMBL3349141CHEMBL201762... |
| P31214 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2 | Rattus norvegicus | OrthoDB | FINASTERIDECHEMBL1778071CHEMBL277224FINASTERIDE... |
| P31213 | 3-oxo-5-alpha-steroid 4-dehydrogenase 2 | Homo sapiens | OrthoDB | Finasteride, 3Steroid, 9aSteroid, 9bSteroid, 10a... |
Ortholog Group (OrthoMCL): OG6_103960
Most Similar Human Ortholog: Q9H8P0
TM-align score: 0.81 | RMSD: 2.38
Seq Identity: 0.25 | Length: 254 / 285
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000128039 | steroid 5 alpha-reductase 3 |
Protein Length: 285 | Molecular Weight (kDa): 34.405
UniProt ID(s): C6S3J5
PDB ID(s): None
Isoelectric Point: 9.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001104 | 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal |
| InterPro | IPR039698 | Polyprenol reductase |
| PFam | PF02544 | 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 13 | 9 | 22 |
| disruptive | 8 | 32 | 20 | 40 |
| missense | 8 | 32 | 18 | 39 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 28 | 19 | 21 |
| disruptive | 14 | 77 | 33 | 81 |
| missense | 12 | 66 | 31 | 60 |
PlasmoDB Total SNPs: 111
Non-coding: 90 | Synonymous: 10 | Nonsynonymous: 11 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 32764679 | Metabolomics profiling reveals new aspects of dolichol biosynthesis in Plasmodiumfalciparum. | Zimbres FM, Valenciano AL, ..., Cassera MB | 10.1038/s41598-020-70246-0 |