Genome location: Pf3D7_14_v3:2,329,251..2,332,697(-)
Genome classification: Core
Product Description: V-type H(+)-translocating pyrophosphatase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 16
EC Numbers: 3.6.1.1 (Inorganic diphosphatase);7.1.3.1 (H(+)-exporting diphosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Function | GO:0015078 | proton transmembrane transporter activity |
| Function | GO:0009678 | pyrophosphate hydrolysis-driven proton transmembrane transporter activity |
| Process | GO:0006885 | regulation of pH |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 1.47 | 0.38 |
| Trophozoite | expressed | 1.00 | 0.50 |
| Schizont | expressed | 0.27 | 0.82 |
| Gametocyte | not expressed | 0.31 | 0.83 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1456800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.238 | MFS: -2.573 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1449800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1320500)
Modification: Disrupted | RMgm-3506
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKR1
"Best" AlphaFill ligand hit: 1PG (2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol, Local RMSD=0.11) with 6AFV (Global RMSD=2.85)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8MTZ4 | H(+)-exporting diphosphatase | Trypanosoma brucei | OrthoMCL BLAST | CHEMBL198409CHEMBL196254CHEMBL54925CHEMBL102478... |
Protein Length: 717 | Molecular Weight (kDa): 76.417
UniProt ID(s): O97154, Q8IKR1, Q9GRI4, Q9UAS4
PDB ID(s): None
Isoelectric Point: 6.48
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004131 | Pyrophosphate-energised proton pump |
| PFam | PF03030 | Pyrophosphate-energised proton pump |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 64 | 22 | 56 |
| disruptive | 3 | 32 | 23 | 57 |
| missense | 3 | 30 | 20 | 53 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 99 | 42 | 28 |
| disruptive | 8 | 99 | 56 | 152 |
| missense | 7 | 70 | 50 | 111 |
PlasmoDB Total SNPs: 95
Non-coding: 63 | Synonymous: 25 | Nonsynonymous: 7 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 11378198 | Two classes of plant-like vacuolar-type H(+)-pyrophosphatases in malariaparasites. | McIntosh MT, Drozdowicz YM, ..., Vaidya AB | 10.1016/s0166-6851(01)00251-1 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |