Genome location: Pf3D7_14_v3:2,336,071..2,339,501(-)
Genome classification: Core
Product Description: signal peptide peptidase
SignalP Peptide: N/A
# Transmembrane Domains: 8
EC Numbers: 3.4.21.89 (Signal peptidase I)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0071458 | integral component of cytoplasmic side of endoplasmic reticulum membrane |
| Component | GO:0071556 | integral component of lumenal side of endoplasmic reticulum membrane |
| Component | GO:0020009 | microneme |
| Function | GO:0042500 | aspartic endopeptidase activity, intramembrane cleaving |
| Function | GO:0008233 | peptidase activity |
| Function | GO:0042803 | protein homodimerization activity |
| Process | GO:0033619 | membrane protein proteolysis |
| Process | GO:0009410 | response to xenobiotic stimulus |
| Process | GO:0006465 | signal peptide processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.52 | 0.77 |
| Trophozoite | expressed | 1.04 | 0.47 |
| Schizont | expressed | 0.77 | 0.56 |
| Gametocyte | expressed | 0.81 | 0.57 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1457000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.767 | MFS: -2.528 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1320700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IKQ9
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.21.89 | Signal peptidase I | NEMSDSEDTAMG132cholateantipainL685,458rifampicinBAL0019193arylomycinlactacystindeoxycholate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102328
Most Similar Human Ortholog: A0A0S2Z6F0
TM-align score: 0.84 | RMSD: 2.20
Seq Identity: 0.38 | Length: 307 / 412
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000101294 | histocompatibility minor 13 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 22 | 13 | 30 |
| disruptive | 4 | 13 | 4 | 33 |
| missense | 4 | 13 | 4 | 28 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 34 | 33 | 25 |
| disruptive | 5 | 46 | 19 | 92 |
| missense | 4 | 36 | 16 | 65 |
PlasmoDB Total SNPs: 240
Non-coding: 218 | Synonymous: 17 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 412 | Molecular Weight (kDa): 47.579
UniProt ID(s): C0KWU7, C0KWV2, Q8IKQ9
PDB ID(s): None
Isoelectric Point: 9.06
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006639 | Presenilin/signal peptide peptidase |
| InterPro | IPR007369 | Peptidase A22B, signal peptide peptidase |
| PFam | PF04258 | Peptidase A22B, signal peptide peptidase |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18160114 | A Presenilin-like protease associated with Plasmodium falciparum micronemes isinvolved in erythrocyte invasion. | Li X, Chen H, Oh SS, Chishti AH | 10.1016/j.molbiopara.2007.11.007 |
| 22844982 | Malaria parasite signal peptide peptidase is an ER-resident protease required forgrowth but not for invasion. | Marapana DS, Wilson DW, ..., Baum J | 10.1111/j.1600-0854.2012.01402.x |
| 25017910 | Plasmodium falciparum signal peptide peptidase cleaves malaria heat shock protein101 (HSP101). Implications for gametocytogenesis. | Baldwin M, Russo C, Li X, Chishti AH | 10.1016/j.bbrc.2014.07.013 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 29187532 | Characterization of SPP inhibitors suppressing propagation of HCV and protozoa. | Hirano J, Okamoto T, ..., Matsuura Y | 10.1073/pnas.1712484114 |
| 38430291 | Characterization and Biological Activities of the Ulvan Polysaccharide-RichFraction Obtained from Ulva rigida and Ulva pseudorotundata and Their Potentialfor Pharmaceutical Application | Favero Massocato T, Robles-Carnero V, ..., Lopez Figueroa FD | 10.1007/s10126-024-10299-8 |